典型文献
Establishment of organoid models based on a nested array chip for fast and reproducible drug testing in colorectal cancer therapy
文献摘要：
The conventional microwell-based platform for construction of organoid models exhibits limitations in precision oncology applications because of low-speed growth and high variability.Here,we established organoid models on a nested array chip for fast and reproducible drug testing using 50％matrigel.First,we constructed mouse small intestinal and colonic organoid models.Compared with the conventional microwell-based platform,the mouse organoids on the chip showed accelerated growth and improved reproducibility due to the nested design of the chip.The design of the chip provides miniaturized and uniform shaping of the matrigel that allows the organoid to grow in a concentrated and controlled manner.Next,a patient-derived organoid(PDO)model from colorectal cancer tissues was successfully generated and characterized on the chip.Finally,the PDO models on the chip,from three patients,were implemented for high-throughput drug screening using nine treatment regimens.The drug sensitivity testing on the PDO models showed good quality control with a coefficient of variation under 10％and a Z'factor of more than 0.7.More importantly,the drug responses on the chip recapitulate the heterogeneous response of individual patients,as well as showing a potential correlation with clinical outcomes.Therefore,the organoid model coupled with the nested array chip platform provides a fast and reproducible means for predicting drug responses to accelerate precise oncology.
文献关键词：
中图分类号：
作者姓名：
Yancheng Cui;Rongrong Xiao;Yushi Zhou;Jianchuang Liu;Yi Wang;Xiaodong Yang;Zhanlong Shen;Bin Liang;Kai Shen;Yi Li;Geng Xiong;Yingjiang Ye;Xiaoni Ai
作者机构：
Department of Gastrointestinal Surgery,Peking University People's Hospital,Beijing 100044,China;Beijing Daxiang Biotech Co.,Ltd.,Beijing 100195,China;Merck Innovation Hub(Guangdong)Co.,Ltd.,Guangzhou 510005,China;State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China
文献出处：
引用格式：
[1]Yancheng Cui;Rongrong Xiao;Yushi Zhou;Jianchuang Liu;Yi Wang;Xiaodong Yang;Zhanlong Shen;Bin Liang;Kai Shen;Yi Li;Geng Xiong;Yingjiang Ye;Xiaoni Ai-.Establishment of organoid models based on a nested array chip for fast and reproducible drug testing in colorectal cancer therapy)[J].生物设计与制造（英文）,2022(04):674-686
A类：
microwell
B类：
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AB值：
0.469705
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