典型文献
A neurovascular unit-on-a-chip: culture and differentiation of human neural stem cells in a three-dimensional microfluidic environment
文献摘要：
Biological studies typically rely on a simple monolayer cell culture, which does not reflect the complex functional characteristics of human tissues and organs, or their real response to external stimuli. Microfluidic technology has advantages of high-throughput screening, accurate control of the fluid velocity, low cell consumption, long-term culture, and high integration. By combining the multipotential differentiation of neural stem cells with high throughput and the integrated characteristics of microfluidic technology, an in vitro model of a functionalized neurovascular unit was established using human neural stem cell-derived neurons, astrocytes, oligodendrocytes, and a functional microvascular barrier. The model comprises a multi-layer vertical neural module and vascular module, both of which were connected with a syringe pump. This provides controllable conditions for cell inoculation and nutrient supply, and simultaneously simulates the process of ischemic/hypoxic injury and the process of inflammatory factors in the circulatory system passing through the blood-brain barrier and then acting on the nerve tissue in the brain. The in vitro functionalized neurovascular unit model will be conducive to central nervous system disease research, drug screening, and new drug development.
文献关键词：
中图分类号：
作者姓名：
Wen-Juan Wei;Ya-Chen Wang;Xin Guan;Wei-Gong Chen;Jing Liu
作者机构：
Stem Cell Clinical Research Center,National Joint Engineering Laboratory,Regenerative Medicine Center,the First Affiliated Hospital of Dalian Medical University,Dalian,Liaoning Province,China;Dalian Innovation Institute of Stem Cell and Precision Medicine,Dalian,Liaoning Province,China
文献出处：
引用格式：
[1]Wen-Juan Wei;Ya-Chen Wang;Xin Guan;Wei-Gong Chen;Jing Liu-.A neurovascular unit-on-a-chip: culture and differentiation of human neural stem cells in a three-dimensional microfluidic environment)[J].中国神经再生研究（英文版）,2022(10):2260-2266
A类：
multipotential
B类：
neurovascular,unit,chip,culture,differentiation,human,neural,cells,three,dimensional,microfluidic,environment,Biological,studies,typically,rely,simple,monolayer,which,does,not,reflect,complex,characteristics,tissues,organs,their,real,response,external,stimuli,Microfluidic,technology,has,advantages,high,throughput,screening,accurate,velocity,low,consumption,long,term,integration,By,combining,integrated,vitro,model,functionalized,was,established,using,derived,neurons,astrocytes,oligodendrocytes,microvascular,barrier,comprises,vertical,module,both,were,connected,syringe,pump,This,provides,controllable,conditions,inoculation,nutrient,supply,simultaneously,simulates,process,ischemic,hypoxic,injury,inflammatory,factors,circulatory,system,passing,blood,brain,then,acting,nerve,will,be,conducive,central,nervous,disease,research,drug,new,development
AB值：
0.612515
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
