典型文献
A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching
文献摘要：
Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and rice.Gold nanoparticles were functionalized with monoclonal antibodies against the tested OPs.DNA oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal amplification.The detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the fluorophore.The resulting fluorescence signal en-ables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concen-tration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,respectively.The mean recovery ranged between 80.3％and 110.8％with relative standard deviations of 7.3％-17.6％,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.
文献关键词：
中图分类号：
作者姓名：
Lingyuan Xu;Xiuyuan Zhang;A.M.Abd El-Aty;Yuanshang Wang;Zhen Cao;Huiyan Jia;J.-Pablo Salvador;Ahmet Hacimuftuoglu;Xueyan Cui;Yudan Zhang;Kun Wang;Yongxin She;Fen Jin;Lufei Zheng;Baima Pujia;Jing Wang;Maojun Jin;Bruce D.Hammock
作者机构：
Institute of Quality Standard and Testing Technology for Agro-Products,Chinese Academy of Agricultural Sciences,Beijing,100081,China;Department of Pharmacology,Faculty of Veterinary Medicine,Cairo University,Giza,12211,Egypt;Department of Medical Pharmacology,Medical Faculty,Ataturk University,Erzurum,25240,Türkiye;Institute of Livestock and Poultry,Ningbo Academy of Agricultural Sciences,Ningbo,Zhejiang,315040,China;Nanobiotechnology for Diagnostics Group,Instituto de Química Avanzada de Catalu?a,IQAC-CSIC,Barcelona,08034,Spain;CIBER de Bioingeniería,Biomateriales y Nanomedicina(CIBER-BBN),Madrid,28029,Spain;Inspection and Testing Center of Agricultural and Livestock Products of Tibet,Department of Agriculture and Rural Affairs of Tibet Autonomous Region,Lhasa,850000,China;Department of Entomology&Nematology and the UC Davis Comprehensive Cancer Center,University of California,Davis,CA,95616,USA
文献出处：
引用格式：
[1]Lingyuan Xu;Xiuyuan Zhang;A.M.Abd El-Aty;Yuanshang Wang;Zhen Cao;Huiyan Jia;J.-Pablo Salvador;Ahmet Hacimuftuoglu;Xueyan Cui;Yudan Zhang;Kun Wang;Yongxin She;Fen Jin;Lufei Zheng;Baima Pujia;Jing Wang;Maojun Jin;Bruce D.Hammock-.A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching)[J].药物分析学报（英文）,2022(04):637-644
A类：
triazophos,parathion,turnips,cabbages,complementarily
B类：
highly,sensitive,bio,barcode,immunoassay,detection,organophosphate,pesticides,fluorescence,quenching,Balancing,risks,benefits,OPs,human,environmental,health,relies,partly,their,accurate,measurement,was,developed,chlorpyrifos,apples,rice,Gold,nanoparticles,were,functionalized,monoclonal,antibodies,against,tested,oligonucleotides,hybridized,fluorescent,label,amplification,signals,generated,by,hybridization,ribonuclease,dissociation,fluorophore,resulting,ables,multiplexed,quantification,residues,concen,tration,limits,respectively,mean,recovery,ranged,between,relative,standard,deviations,which,correlate,well,results,obtained,liquid,chromatography,tandem,mass,spectrometry,LC,proposed,stable,reproducible,reliable,low,residual,levels,agricultural,products
AB值：
0.526935
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
