典型文献
Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products
文献摘要：
In this study, a label-free, portable and reproducible immunochip based on quartz crystal microbalance (QCM) was developed for the qualitative detection of β-lactoglobulin (β-LG), an allergen, in milk products. Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment, amount of anti-β-LG antibody and regeneration reagent were optimized in detail. Under optimal conditions, the proposed QCM immunochip exhibited good recognition of β-LG, with a calibration curve of ΔF = 12.877Cβ-LG0.4809 (R2 = 0.9982) and limit of detection of 0.04 μg/mL. Additionally, this portable QCM immunochip had good stability, high specificity, and no obvious cross-reaction to three other milk proteins (α-casein, α-lactalbumin, and lactoferrin). It could compete a qualitative measurement within 5 min, and could be reused at least ten times. In the β-LG analysis of actual milk samples, the developed QCM immunochip yielded reliable and accurate results, which correlated strongly with those from the standard HPLC method (R2 = 0.9969). Thus, the portable, stable, and reproducible QCM immunochip developed in this study allowed the rapid, cost-effectively and sensitively measure the β-LG in milk products.
文献关键词：
中图分类号：
作者姓名：
Mingfei Pan;Liping Hong;Jingying Yang;Xiaoqian Xie;Kaixin Liu;Shuo Wang
作者机构：
Key Laboratory of Food Nutrition and Safety,Ministry of Education of China,Tianjin University of Science and Technology,Tianjin 300457,China;State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457,China
文献出处：
引用格式：
[1]Mingfei Pan;Liping Hong;Jingying Yang;Xiaoqian Xie;Kaixin Liu;Shuo Wang-.Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products)[J].食品科学与人类健康（英文）,2022(05):1315-1321
A类：
immunochip,877C
B类：
Fabrication,evaluation,portable,reproducible,quartz,crystal,microbalance,label,free,detection,lactoglobulin,allergen,milk,products,In,this,study,QCM,was,developed,qualitative,Experimental,parameters,fabrication,regeneration,procedure,such,coupling,microenvironment,amount,antibody,reagent,were,optimized,detail,Under,optimal,conditions,proposed,exhibited,good,recognition,calibration,curve,LG0,limit,Additionally,had,stability,high,specificity,obvious,cross,reaction,three,other,proteins,casein,lactalbumin,lactoferrin,It,could,compete,measurement,within,reused,least,ten,times,analysis,actual,samples,yielded,reliable,accurate,results,which,correlated,strongly,those,from,standard,HPLC,method,Thus,stable,allowed,rapid,cost,effectively,sensitively
AB值：
0.51866
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