典型文献
Thrombospondin 1 Promotes Endoplasmic Reticulum Stress and Apoptosis in HK-2 Cells by Upregulating ATF6-CHOP
文献摘要：
Objective:The goal of this study is to investigate the role and mechanism of endoplasmic reticulum stress and apoptosis regulated by thrombospondin 1(TSP1)in human renal tubular epithelial cells(HK-2 cells).Methods:HK-2 cells were exposed to high concentrations of glucose(HG).The endoplasmic reticulum stress inhibitor 4-phenylbutyric acid(4-PBA)was administered by transfecting TSP1 or an empty vector to explore the mechanism of the endoplasmic reticulum response regulated by TSP1 and stress in renal cell apoptosis.The effects of TSP1 and 4-PBA on the proliferation and apoptosis of HK-2 cells under HG conditions were assessed using Cell counting kit-8 and flow cytometry.Western blotting was used to detect the apoptosis-and endoplasmic reticulum stress-related protein expression regulated by TSP1 and 4-PBA.Results:HG treatment induced high cell apoptosis,abundantly expressed TSP1 level and restrained viability in HK-2 cells.Overexpression of TSP1 significantly inhibited the proliferation of and facilitated apoptosis of HK-2 cells under HG conditions.Administration of endoplasmic reticulum stress inhibitor 4-PBA after overexpression of TSP1 antagonized the inhibitory proliferation and promoted apoptosis rate in HG-triggered HK-2 cells induced by TSP1 overexpression.4-PBA treatment significantly hindered the expression of endoplasmic reticulum stress markers,such as PERK,ATF4,ATF6,p-eIF2α,IRE1,CHOP and XBP1,suggesting that the administration of 4-PBA was successful.Conclusion:Overexpression of TSP1 activated endoplasmic reticulum stress by regulating the ATF6-CHOP axis.TSP1 restrained cell proliferation,and promoted apoptosis and endoplasmic reticulum stress by activating the ATF6-CHOP axis.
文献关键词：
中图分类号：
作者姓名：
Li-li YUE;Xin DU
作者机构：
Department of Nephrology,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,China
文献出处：
引用格式：
[1]Li-li YUE;Xin DU-.Thrombospondin 1 Promotes Endoplasmic Reticulum Stress and Apoptosis in HK-2 Cells by Upregulating ATF6-CHOP)[J].当代医学科学(英文),2022(02):341-347
A类：
Thrombospondin,Upregulating
B类：
Promotes,Endoplasmic,Reticulum,Stress,Apoptosis,HK,Cells,by,ATF6,CHOP,Objective,goal,this,study,investigate,role,mechanism,endoplasmic,reticulum,stress,apoptosis,regulated,thrombospondin,TSP1,human,renal,tubular,epithelial,cells,Methods,were,exposed,high,concentrations,glucose,HG,phenylbutyric,acid,PBA,was,administered,transfecting,empty,vector,explore,response,effects,proliferation,under,conditions,assessed,using,counting,kit,flow,cytometry,blotting,used,detect,related,protein,Results,treatment,induced,abundantly,expressed,level,restrained,viability,Overexpression,significantly,inhibited,facilitated,Administration,after,overexpression,antagonized,inhibitory,promoted,rate,triggered,hindered,markers,such,PERK,ATF4,eIF2,IRE1,XBP1,suggesting,that,administration,successful,Conclusion,activated,axis,activating
AB值：
0.432857
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
