Objective:To elucidate the mechanisms of 4 effective components from a Chinese medicine formula,namely Qingre Huoxue Jiedu Formula(QHJ heat-and toxin-clearing and blood-activating formula),in the treatment of nerve growth factor(NGF)-induced psoriasis.Methods:Keratinocyte proliferation and T cell proliferation models were developed using NGF.An NGF solution(NGF+DMEM,100 ng/mL)was added to all induced groups and treated groups and were cultured for 24 h,while a solution with NTRK1 antagonist(K252a+DEME,300 nmol/L)was added and cultured for 1 h.The models were used to evaluate the effects of the treatment with each of the 4 components of QHJ,namely shikonin,paeonol,astilbin and ursolic acid.Cell apoptosis and proliferation were measured by flow cytometry analysis and CCK8 assay,respectively.The mRNA expression levels of Bax,Bcl-xl,and NGF receptor(NGFR)were assessed by quantitative real-time PCR(qRT-PCR)and Western blot analysis,respectively.Results:(1)All QHJ-treated groups showed significantly increased cell apoptosis and inhibition of cell proliferation compared with the NGF-induced groups(P＜0.05).In addition,treatment with QHJ plus NTRK1 significantly enhanced cell apoptosis and inhibition of cell proliferation compared with cells treated with QHJ only(P＜0.05),particularly in cells treated with ursolic acid.(2)QHJ-treated groups showed higher protein expression levels of Bax,Bcl-xl compared with other groups(P＜0.05).Additionally,treatment with QHJ plus NTRK1 significantly increased the protein expression levels of Bax,Bcl-xl and NGFR compared with those treated with QHJ only(all P＜0.05),especially in those treated with shikonin.Conclusion:The action mechanism of QHJ on psoriasis might be through enhancing cell apoptosis and inhibition of cell proliferation,and upregulating the expression level of Bax,Bcl-xl and NGFR.

WANG Jun-hui;JIANG Ying-juan;LI Min;WANG Ning;CUI Bing-nan;LIU Wa-li

Dermatology Department,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Dermatology Department,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Laboratory of Molecular Biology,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China

中国结合医学杂志（英文版）

[1]WANG Jun-hui;JIANG Ying-juan;LI Min;WANG Ning;CUI Bing-nan;LIU Wa-li-.Effects of Qingre Huoxue Jiedu Formula on Nerve Growth Factor-Induced Psoriasis)[J].中国结合医学杂志（英文版）,2022(03):236-242

QHJ,Keratinocyte,NGF+DMEM,K252a+DEME,astilbin

Effects,Qingre,Huoxue,Jiedu,Formula,Nerve,Growth,Factor,Induced,Psoriasis,Objective,To,elucidate,mechanisms,effective,components,from,Chinese,medicine,formula,namely,heat,toxin,clearing,blood,activating,treatment,nerve,growth,induced,psoriasis,Methods,proliferation,models,were,developed,using,An,solution,was,added,groups,treated,cultured,while,NTRK1,antagonist,nmol,used,evaluate,effects,each,shikonin,paeonol,ursolic,acid,Cell,apoptosis,measured,by,flow,cytometry,analysis,CCK8,assay,respectively,expression,levels,Bax,Bcl,xl,receptor,NGFR,assessed,quantitative,real,qRT,blot,Results,All,showed,significantly,increased,inhibition,compared,addition,plus,enhanced,cells,only,particularly,higher,protein,other,Additionally,those,especially,Conclusion,action,might,be,through,enhancing,upregulating

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