Objective:To determine whether salvianolic acid B(Sal B)exerts protective effects on diabetic peripheral neuropathy by attenuating apoptosis and pyroptosis.Methods:RSC96 cells were primarily cultured with DMEM(5.6 mmol/L glucose),hyperglycemia(HG,125 mmol/L glucose)and Sal B(0.1,1,and 10 μmol/L).Cells proliferation was measured by 3-(4,5-cimethylthiazol-2-yl)-2,5-dilphenyltetrazolium bromide assay.Reactive oxygen species(ROS)generation and apoptosis rate were detected by flow cytometry analysis.Western blot was performed to analyze the expressions of poly ADP-ribose polymerase(PARP),cleaved-caspase 3,cleaved-caspase 9,Bcl-2,Bax,NLRP3,ASC,and interleukin(IL)-1 β.Results:Treatment with HG at a concentration of 125 mmol/L attenuated cellular proliferation,while Sal B alleviated this injury(P＜0.05).In addition,Sal B inhibited HG-induced ROS production and apoptosis rate(P＜0.05).Furthermore,treatment with Sal B down-regulated HG-induced PARP,cleaved-caspase 3,cleaved-caspase 9,Bax,NLRP3,ASC,and IL-1 β expression,but mitigated HG-mediated down-regulation of Bcl-2 expression(P＜0.05).Conclusion:Sal B may protect RSC96 cells against HG-induced cellular injury via the inhibition of apoptosis and pyroptosis activated by ROS.

Department of Traditional Chinese Medicine,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China;Department of Peripheral Vascular,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China

[1]WANG Qian-qian;WANG Meng;LI Yan;LIU Yong-hui;SUN Lian-qing-.Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B)[J].中国结合医学杂志（英文版）,2022(03):243-248

cimethylthiazol,dilphenyltetrazolium

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