Objective:To study the protective effect of anthocyanins extracted from Vaccinium Uliginosum(VU)on retinal 661W cells against microwave radiation induced retinal injury.Methods:661W cells were divided into 6 groups,including control,model[661W cells radiated by microwave(30 mW/cm2,1 h)]and VU groups[661W cells pretreated with anthocyanins extracted from VU(25,50,100 and 200 μg/mL,respectively)for 48 h,and radiated by microwave 30 mW/cm2,1 h].After treatment with different interventions,the cell apoptosis index(Al)was determined using Heochst staining;contents of malonaldehyde(MDA),glutataione(GSH),and activity of superoxide dismutase(SOD)were measured.mRNA expressions of nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase 1(HO-1)were detected by real time quantitative polymerase chain reaction,and the expression of HO-1 protein was examined by Western blot analysis.Nucleus and cytoplasm were separated and Nrf2 protein expression was further verified by Western blot analysis.Results:There was significant difference in Al among the groups(F=322.83,P＜0.05).Compared with the control group,Al was significantly higher in the model group and was lower in 4 VU-pretreated groups(P＜0.05).Linear regression analysis showed the decline of Al was in a dose-dependent manner with VU treatment(r=0.8419,P＜0.05).The MDA and GSH contents of 661W cells in VU-treated groups were significantly lower than the model group(P＜0.05).Compared with the model group,the SOD activity in the VU-treated groups(50,100 and 200 μg/mL)was significantly higher(all P＜0.05).The Nrf2 and HO-1 mRNA expressions were slightly increased after irradiation,and obviously increased in 100 μg/mL VU-treated group.After irradiation,the relative expressions of HO-1 and Nrf2 proteins in nucleus were slightly increased(P＜0.05),and the changes in cytoplasm were not obvious,whereas it was significantly increased in both nucleus and cytoplasm in the VU treatment groups.Conclusions:Anthocyanins extracted from VU could reduce apoptosis,stabilize cell membrane,and alleviate oxidant injury of mouse retinal photoreceptor 661W cells.The mechanism might be through activating Nrf2/HO-1 signal pathway and inducing HO-1 transcription and translation.

YIN Lan;FAN Si-jun;ZHANG Mao-nian

Department of Ophthalmology,Chinese PLA General Hospital,Beijing,100853,China;Department of Ophthalmology,PLA Rocket Characteristic Medical Center,Beijing,100853,China

中国结合医学杂志（英文版）

[1]YIN Lan;FAN Si-jun;ZHANG Mao-nian-.Protective Effects of Anthocyanins Extracted from Vaccinium Uliginosum on 661W Cells Against Microwave-Induced Retinal Damage)[J].中国结合医学杂志（英文版）,2022(07):620-626

Extracted,Uliginosum,Heochst

Protective,Effects,Anthocyanins,from,Vaccinium,661W,Cells,Against,Microwave,Induced,Retinal,Damage,Objective,To,study,protective,effect,anthocyanins,extracted,VU,retinal,cells,against,microwave,induced,injury,Methods,were,divided,into,groups,including,control,model,radiated,by,mW,pretreated,respectively,After,treatment,different,interventions,apoptosis,was,determined,using,staining,contents,malonaldehyde,glutataione,GSH,activity,superoxide,dismutase,measured,expressions,nuclear,erythroid,related,Nrf2,heme,oxygenase,HO,detected,real,quantitative,polymerase,chain,reaction,examined,blot,analysis,Nucleus,cytoplasm,separated,further,verified,Results,There,difference,among,Compared,significantly,higher,lower,Linear,regression,showed,decline,dose,dependent,manner,than,slightly,increased,after,irradiation,obviously,relative,proteins,nucleus,changes,not,whereas,both,Conclusions,could,reduce,stabilize,membrane,alleviate,oxidant,mouse,photoreceptor,mechanism,might,be,through,activating,signal,pathway,inducing,transcription,translation

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