Objective Vitamin D and Toll-like receptor-4 (TLR-4) inhibition are involved in the protection of keratinocytes. The effects of combination of 1,25(OH)2D3 and TLR-4 inhibitor on the protection of keratinocytes against ultraviolet radiation B (UVB) irradiation remain unclear. This study was undertaken to explore the effects of combination of 1,25(OH)2D3 and TAK-242 (TLR-4 inhibitor) on the damage to HaCaT cells caused by UVB irradiation.Methods In vitro, HaCaT cells were treated with 1,25(OH)2D3 or/and TAK-242 prior to UVB irradiation at the intensity of 20 mJ/cm2, then the production of reactive oxygen species (ROS), cell migration, apoptosis of cells, and the expression of oxidative stress, endoplasmic reticulum stress, and apoptosis related proteins were determined. Results Compared with the HaCaT cells treated with 1,25(OH)2D3 or TAK-242, the cells treated with both 1,25(OH)2D3 and TAK-242 showed, 1) significantly lower production of ROS (P < 0.05);2) significantly less apoptosis of HaCaT cells (P < 0.05); 3) significantly lower expression of NF-κB, Caspase-8, Cyto-C, Caspase-3 (P < 0.05). Conclusion The combination of 1,25(OH)2D3 and TAK-242 could produce a better protection for HaCaT cells via inhibiting the oxidative stress, endoplasmic reticulum stress and apoptosis than 1,25(OH)2D3 or TAK-242 alone.

CHEN Peng;ZHUANG Chuan Ning;CUI Jia Jing;WANG Ping Wei;LIU Dong Ge;YAN Shu Qi;ZHOU Li Ting;REN Shu Ping

Department of Pediatrics,the Second Hospital of Jilin University,Changchun 130041,Jilin,China;Department of Occupational and Environmental Health,School of Public Health,Jilin University,Changchun 130021,Jilin,China

生物医学与环境科学（英文版）

[1]CHEN Peng;ZHUANG Chuan Ning;CUI Jia Jing;WANG Ping Wei;LIU Dong Ge;YAN Shu Qi;ZHOU Li Ting;REN Shu Ping-.Effects of Combination of 1,25(OH)2D3 and TLR-4 Inhibitor on the Damage to HaCaT Cells Caused by UVB Irradiation)[J].生物医学与环境科学（英文版）,2022(11):1051-1062

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