Objective:To explore the protective effect and underlying mechanism of Lycium barbarum polysaccharides(LBP)in a non-alcoholic fatty liver disease(NAFLD)cell model.Methods:Normal human hepatocyte LO2 cells were treated with 1 mmol/L free fatty acids(FFA)mixture for 24 h to induce NAFLD cell model.Cells were divided into 5 groups,including control,model,low-,medium-and high dose LBP(30,100 and 300 μ g/mL)groups.The monosaccharide components of LBP were analyzed with high performance liquid chromatography.Effects of LBP on cell viability and intracellular lipid accumulation were assessed by cell counting Kit-8 assay and oil red O staining,respectively.Triglyceride(TG),alanine aminotransferase(ALT),aspartate aminotransferase(AST),adenosine triphosphate(ATP)and oxidative stress indicators were evaluated.Energy balance and mitochondrial biogenesis related mRNA and proteins were determined by quantitative real-time polymerase chain reaction and Western blot,respectively.Results:Heteropolysaccharides with mannose and glucose are the main components of LBP.LBP treatment significantly decreased intracellular lipid accumulation as well as TG,ALT,AST and malondialdehyde levels(P＜0.05 or P＜0.01),increased the levels of superoxide dismutase,phospholipid hydroperoxide glutathione peroxidase,catalase,and ATP in NAFLD cell model(P＜0.05).Meanwhile,the expression of uncoupling protein 2 was down-regulated and peroxisome proliferator-activated receptor gamma coactivator-1 α/nuclear respiratory factor 1/mitochondrial transcription factor A pathway was up-regulated(P＜0.05).Conclusion:LBP promotes mitochondrial biogenesis and improves energy balance in NAFLD cell model.

School of Public Health and Management,Ningxia Medical University,Yinchuan 750004,China;Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control,Yinchuan 750004,China;Xi'an Chang'an South Road Community Health Service Center,Xi'an Yanta Hospital of Chinese Medicine,Xi'an 710000,China

[1]ZHANG Yan-nan;GUO Yi-qiong;FAN Yan-na;TAO Xiu-juan;GAO Qing-han;YANG Jian-jun-.Lycium barbarum Polysaccharides Promotes Mitochondrial Biogenesis and Energy Balance in NAFLD Cell Model)[J].中国结合医学杂志（英文版）,2022(11):975-982

Biogenesis,Heteropolysaccharides

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