Objective:To evaluate the protective function of Babao Dan(BBD)on 5-flurouracil(5-FU)-induced intestinal mucositis(IM)and uncover the underlying mechanism.Methods:A total of 18 male mice were randomly divided into 3 groups by a random number table,including control,5-FU and 5-FU combined BBD groups,6 mice in each group.A single intraperitoneal injection of 5-FU(150 mg/kg)was performed in 5-FU and 5-FU combined BBD groups on day 0.Mice in 5-FU combined BBD group were gavaged with BBD(250 mg/kg)daily from day 1 to 6.Mice in the control group were gavaged with saline solution for 6 days.The body weight and diarrhea index of mice were recorded daily.On the 7th day,the blood from the heart of mice was collected to analyze the proportional changes of immunological cells,and the mice were subsequently euthanized by mild anesthesia with 2％pentobarbital sodium.Colorectal lengths and villus heights were measured.Intestinal-cellular apoptosis and proliferation were evaluated by Tunel assay and immunohistochemical staining of proliferating cell nuclear antigen,respectively.Immunohistochemistry and Western blot were performed to investigate the expressions of components in Wnt/β-catenin pathway(Wnt3,LRP5,β-catenin,c-Myc,LRG5 and CD44).Results:BBD obviously alleviated 5-FU-induced body weight loss and diarrhea,and reversed the decrease in the number of white blood cells,including monocyte,granulocyte and lymphocyte,and platelet(P＜0.01).The shortening of colon caused by 5-FU was also reversed by BBD(P＜0.01).Moreover,BBD inhibited apoptosis and promoted proliferation in jejunum tissues so as to reduce the intestinal mucosal damage and improve the integrity of villus and crypts.Mechanically,the expression levels of Wnt/β-catenin mediators such as Wnt3,LRP5,β-catenin were upregulated by BBD,activating the transcription of c-Myc,LRG5 and CD44(P＜0.01).Conclusions:BBD attenuates the adverse effects induced by 5-FU via Wnt/β-catenin pathway,suggesting it may act as a potential agent against chemotherapy-induced intestinal mucositis.

GUI Meng-xuan;HUANG Bin;PENG Jun;CHEN Xi;Ragunath Muthu;GAO Ying;WANG Rui-guo;LIN Jiu-mao

Academy of Integrative Medicine,Fujian Key Laboratory of Integrative Medicine on Geriatrics,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Department of Medical Oncology,900 Hospital of the Joint Logistics Team Support Force,Fuzhou 350025,China

中国结合医学杂志（英文版）

[1]GUI Meng-xuan;HUANG Bin;PENG Jun;CHEN Xi;Ragunath Muthu;GAO Ying;WANG Rui-guo;LIN Jiu-mao-.Babao Dan Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway)[J].中国结合医学杂志（英文版）,2022(11):1000-1006

Babao,flurouracil,pentobarbital,LRG5

Dan,Alleviates,Fluorouracil,Induced,Intestinal,Damage,Catenin,Pathway,Objective,To,protective,function,BBD,FU,induced,intestinal,mucositis,IM,uncover,underlying,mechanism,Methods,total,male,mice,were,randomly,divided,into,groups,by,number,table,including,control,combined,each,single,intraperitoneal,injection,was,performed,Mice,gavaged,daily,from,saline,solution,days,body,weight,diarrhea,recorded,On,7th,blood,heart,collected,analyze,proportional,changes,immunological,cells,subsequently,euthanized,mild,anesthesia,sodium,Colorectal,lengths,villus,heights,measured,cellular,apoptosis,proliferation,evaluated,Tunel,assay,immunohistochemical,staining,proliferating,nuclear,antigen,respectively,Immunohistochemistry,blot,investigate,expressions,components,catenin,pathway,Wnt3,LRP5,Myc,CD44,Results,obviously,alleviated,loss,reversed,decrease,white,monocyte,granulocyte,lymphocyte,platelet,shortening,colon,caused,also,Moreover,inhibited,promoted,jejunum,tissues,reduce,mucosal,damage,improve,integrity,crypts,Mechanically,levels,mediators,such,upregulated,activating,transcription,Conclusions,attenuates,adverse,effects,suggesting,may,potential,agent,against,chemotherapy

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