It is necessary to develop a new strategy for treatment of lung cancer since it is the main cause of cancer death.Tanshinone ⅡA(Tan ⅡA),an active ingredient of a commonly used traditional Chinese herb Salvia miltiorrhiza,provides a new direction to develop a new strategy for the treatment.It has been found that Tan ⅡA could inhibit lung cancer in vitro and in vivo by inducing autophagic apoptosis.Tan ⅡA increased apoptotic cells and the expression of cleaved caspase 3 and cleaved caspase 9,decreased B-cell lymphoma-2(Bcl-2)/Bcl-2 associated X protein(Bax)ratio in human non-small cell lung cancer(NSCLC)cell lines,which was promoted by an autophagy activator Rapamycin,and weaken by autophagy inhibitor 3-methyladenine(3-MA).In addition,Tan ⅡA induced more autophagosomes,up-regulated light chain 3β(LC-3B)Ⅰ and LC-3B Ⅱ conversion and less sequestosome 1(SQSTM1/p62)expression,which cannot be weakened by the caspase 3 antagonist.Moreover,overexpression of LC-3B gene(LC3B)and downregulation of autophagic gene 5(ATG5)further confirmed that Tan ⅡA induced autopha-gic apoptosis in NSCLC cell lines.Beclin-1 gene(BECN1)overexpression and silence attenuated the effects of Tan ⅡA,suggesting autophagic apoptosis that Tan ⅡA induced was dependent on Beclin-1.Overall,our study demonstrated a new treatment mechanism of Tan ⅡA and suggested that Tan ⅡA is a potential new anti-cancer therapeutic option.

Shasha Bai;Sainan Cui;Wenhao Wen;Elaine Lai-Han Leung;Jing Bai;Huiyuan Lin;Yongfei Cui;Lei Yang;Zhongqiu Liu;Yuan Zheng;Rong Zhang

School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;State Key Laboratory of Quality Research in Chinese Medicine/Macau Institute for Applied Research in Medicine and Health,Macau University of Science and Technology,Macau 999078,China;Cardiothoracic Surgery Department,The Second Clinical College of Guangzhou University of Chinese Medicine,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510006,China

工程（英文）

[1]Shasha Bai;Sainan Cui;Wenhao Wen;Elaine Lai-Han Leung;Jing Bai;Huiyuan Lin;Yongfei Cui;Lei Yang;Zhongqiu Liu;Yuan Zheng;Rong Zhang-.Tanshinone ⅡA Suppresses Non-Small Cell Lung Cancer Through Beclin-1-Mediated Autophagic Apoptosis)[J].工程（英文）,2022(12):128-138

autopha

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