Phenolamide(PA)metabolites play important roles in the interaction between plants and pathogens.The putrescine hydroxycinnamoyl transferase genes OsPHT3 and OsPHT4 positively regulate rice cell death and resistance to Magnaporthe oryzae.The bZIP transcription factor APIP5,a negative regulator of cell death and rice immunity,directly binds to the OsPHT4 promoter to regulate putrescine-derived PAs.Whether other hydroxycinnamoyl transferase(HT)genes also participate in AP/P5-mediated immunity re-mains unclear.Surprisingly,we find that genes encoding agmatine hydroxycinnamoyl transferases OsAHT1 and OsAHT2,tryptamine hydroxycinnamoyl transferases OsTBT1 and OsTBT2,and tyramine hydrox-ycinnamoyl transferases OsTHT1 and OsTHT2,responsible for the biosynthesis of polyamine-derived PAs are all up-regulated in APIP5-RNAitransgenic plants compared with segregated wild-type rice.Further-more,both OsAHT1/2 and OsTBT1/2 are induced during M.oryzae infection,showing expression patterns similar to those previously reported for OsTHT1/2 and OsPHT3/4.Transgenic plants overexpressing either OsAHT2-GFP or OsTBT1-GFP show enhanced resistance against M.oryzae and accumulated more PA metabolites and lignin compared with wild-type plants.Interestingly,as demonstrated for OsPHT4,APIP5 directly binds to the promoters of OsAHT1/2,OsTBT1/2,and OsTHT1/2,repressing their transcription.Together,these results indicate that the HT genes are common targets of APIP5 and that PAs play critical roles in rice immunity.

Hong Fang;Fan Zhang;Chongyang Zhang;Dan Wang;Shuangqian Shen;Feng He;Hui Tao;Ruyi Wang;Min Wang;Debao Wang;Xionglun Liu;Jie Luo;Guo-Liang Wang;Yuese Ning

College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan,Hubei 430070,China;College of Tropical Crops,Hainan University,Haikou,Hainan 572208,China;Department of Plant Pathology,The Ohio State University,Columbus,OH 43210,USA

遗传学报

[1]Hong Fang;Fan Zhang;Chongyang Zhang;Dan Wang;Shuangqian Shen;Feng He;Hui Tao;Ruyi Wang;Min Wang;Debao Wang;Xionglun Liu;Jie Luo;Guo-Liang Wang;Yuese Ning-.Function of hydroxycinnamoyl transferases for the biosynthesis of phenolamides in rice resistance to Magnaporthe oryzae)[J].遗传学报,2022(08):776-786

hydroxycinnamoyl,phenolamides,Phenolamide,OsPHT3,OsPHT4,APIP5,agmatine,OsAHT1,OsAHT2,OsTBT1,OsTBT2,ycinnamoyl,OsTHT1,OsTHT2,RNAitransgenic

Function,transferases,biosynthesis,rice,resistance,Magnaporthe,oryzae,metabolites,play,important,roles,interaction,between,plants,pathogens,putrescine,genes,positively,cell,death,bZIP,transcription,negative,regulator,immunity,directly,binds,derived,PAs,Whether,other,also,participate,mediated,mains,unclear,Surprisingly,find,that,encoding,tryptamine,tyramine,responsible,polyamine,all,up,regulated,compared,segregated,wild,type,Further,more,both,induced,during,infection,showing,expression,patterns,similar,those,previously,reported,Transgenic,overexpressing,either,GFP,enhanced,against,accumulated,lignin,Interestingly,demonstrated,promoters,repressing,their,Together,these,results,indicate,common,targets,critical

0.36898