In order to elucidate the contributions of JA in orchestrating disease resistance in potato plants,the potato genotype'SD20',which exhibits strong resistance against the highly virulent Phytophthora infestans isolate CN152,while infected by the super virulent isolate 2013-18-306,was treated with exogenous JA and then challenged by inoculation with 2013-18-306.The results showed that exogenously applied JA significantly delayed the onset and alleviated the symptoms of late blight,indicating exogenous JA could induce resistance to P.infestans in the early biotrophic stage of infection in'SD20'plants.To further clarify the role of JA in the early defense response and identify key genes involved in JA signal transduction,gene expression profiling via RNA sequencing(RNA-seq)in'SD20'plants treated with exogenously applied JA was performed.A total of 2 927 differentially expressed genes were specifically induced,the majority encoded transcription factors,protein kinases,secondary metabolites,defense enzymes and disease resistance related proteins.GO functional annotation and KEGG metabolic pathway analysis showed that exogenously applied JA rapidly induced the expression of genes related to immune response regulation,pathogen defense,and other biological processes,and stimulated endogenous JA synthesis and signal transduction,and the overall early pathogen defense response in'SD20'.These results provide useful information in understanding the JA's function involved in pathogen defense responses and a theoretical basis for the application of JA in potato production.

Yu Yang;Xiaohui Yang;Xiao Guo;Xinxi Hu;Daofeng Dong;Guangcun Li;Xingyao Xiong

Hunan Provincial Engineering Research Center for Potatoes,Key Laboratory for Vegetable Biology of Hunan Province,College of Horticulture,Hunan Agricultural University,Changsha 410128,China;Institute of Vegetable Research,Shandong Academy of Agricultural Sciences/Molecular Biology Key Laboratory of Shandong Facility Vegetable,Ji'nan 250100,China;Institute of Vegetables and Flowers,Ministry of Agriculture and Rural Affairs,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop,Beijing 100081,China;Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518120,China

园艺学报（英文版）

[1]Yu Yang;Xiaohui Yang;Xiao Guo;Xinxi Hu;Daofeng Dong;Guangcun Li;Xingyao Xiong-.Exogenously applied methyl Jasmonate induces early defense related genes in response to Phytophthora infestans infection in potato plants)[J].园艺学报（英文版）,2022(04):511-526

Exogenously,SD20,CN152,biotrophic

applied,methyl,Jasmonate,induces,early,defense,related,genes,Phytophthora,infestans,infection,potato,plants,In,order,elucidate,contributions,JA,orchestrating,disease,resistance,genotype,which,exhibits,strong,against,highly,virulent,isolate,while,infected,by,super,was,treated,then,challenged,inoculation,results,showed,that,exogenously,significantly,delayed,onset,alleviated,symptoms,blight,indicating,could,stage,To,further,clarify,role,identify,key,involved,signal,transduction,expression,profiling,sequencing,performed,total,differentially,expressed,were,specifically,induced,majority,encoded,transcription,factors,kinases,secondary,metabolites,enzymes,proteins,functional,annotation,metabolic,pathway,analysis,rapidly,immune,regulation,pathogen,other,biological,processes,stimulated,endogenous,synthesis,overall,These,provide,useful,information,understanding,responses,theoretical,basis,application,production

0.483394