Bacterial spot(BS)is a severe bacterial disease induced by Xanthomonas campestris pv.vesicatoria(Xcv),a pathogen that causes serious damage to pepper growth and yield.It is therefore important to study the mechanisms of pepper resistance to Xcv and to breed and promote Xcv-resistant pepper varieties.However,studies of the responses to Xcv infection in peppers at the protein level are limited.Here,we examined Xcv-induced proteomic changes in leaves of the BS susceptible bell pepper ECW and the resistant bell pepper VI037601 using the isobaric tags for relative and absolute quantitation(iTRAQ)-based protein labeling technology.A total of 6,120 distinct proteins were identified,and there were 1,289 significantly differentially accumulated proteins(DAPs)in ECW and VI037601 leaves after Xcv inoculation.Among these,339(250 up-and 89 down-regulated)and 479(300 up-and 179 down-regulated)DAPs were specifically identified in ECW and VI037601,respectively,with 459(364 up-and 95 down-regulated)similarly expressed DAPs being shared by ECW and VI037601.Based on bioinformatics analysis,many defense-associated proteins were identified as up-regulated in ECW and VI037601,especially the proteins involved in plant-pathogen interaction,phenylpropanoid biosynthesis,protein processing in the endoplasmic reticulum,and MAPK signaling pathway-plant.Moreover,we evaluated transcript levels of six differentially expressed genes from the iTRAQ results by qRT-PCR.The analysis revealed transcriptional changes that were consistent with the changes at the protein level.This study will provide a valuable resource for understanding the molecular basis of pepper resistance to Xcv infection and for improving the disease resistance of pepper cultivars.

Hubei Academy of Agricultural Sciences,Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement,Cash Crops Research Institute,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430070,China;Kasetsart University,Bangkok 10900,Thailand

[1]Fei Wang;Shenghua Gao;Juntawong Niran;Ning Li;Yanxu Yin;Chuying Yu;Chunhai Jiao;Minghua Yao-.iTRAQ proteomics reveals the regulatory response to Xanthomonas campestris pv.vesicatoria in resistant vs.susceptible pepper genotypes)[J].园艺学报（英文版）,2022(06):747-756

vesicatoria,Xcv,VI037601

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