Background:Very low-protein (VLP) diets negatively impact calcium (Ca) metabolism and absorption. The objective of this study was to investigate the effect of supplemental branched-chain amino acids (BCAA) and limiting amino acids (LAA) on Ca digestibility, absorption and reabsorption in pigs fed with VLP diets. Forty-eight piglets were assigned to six treatments:positive control (PC), negative control (NC), and NC containing LAA 25％, LAA 50％, LAA+BCAA 25％(LB25) and LAA+BCAA 50％(LB50) more than recommendations. Results:Relative to PC or NC, LB25 and LB50 had higher digestibility of Ca and plasma Ca and phosphorus (P), but lower plasma vitamin D3. LB50 tended to increase vitamin D receptor transcript and protein in the gut, but decreased mRNA or protein abundance of parathyroid hormone 1 receptor (PTH1R), calbindin 1 (CALB1), cytochrome P450 family 27 subfamily B member 1 and occludin in small intestine. LB50 increased the transcript of cytochrome P450 family 24 subfamily A member 1 and PTH1R but decreased the transcript of transient receptor potential cation channel subfamily V member 5, CALB1 and solute carrier family 17 member 4 in kidney. Conclusion:Overall, BCAA increased Ca digestibility through regulating the transcellular and paracellular Ca absorption in the gut and reabsorption in kidney during protein restriction.

Mohammad Habibi;Cedrick N.Shili;Julia Sutton;Parniyan Goodarzi;Adel Pezeshki

Department of Animal and Food Sciences,Oklahoma State University,206C Animal Science Building,Stillwater,OK 74078,USA

畜牧与生物技术杂志（英文版）

[1]Mohammad Habibi;Cedrick N.Shili;Julia Sutton;Parniyan Goodarzi;Adel Pezeshki-.Dietary branched-chain amino acids modulate the dynamics of calcium absorption and reabsorption in protein-restricted pigs)[J].畜牧与生物技术杂志（英文版）,2022(04):1083-1098

LAA+BCAA,LB25,LB50,calbindin,transcellular

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