Our previous studies demonstrated that prenatal in utero growth restriction impairs postnatal intestinal function.Thus,improving postpartal intestinal absorption capacity and growth by manipulating the maternal diet prepartum is of importance.This work was conducted to determine whether supple-mentation of N-carbamylglutamate(NCG)or rumen-protected L-arginine(RP-Arg)increased fetal in-testinal amino acid(AA)profiles in intrauterine growth retardation(IUGR)fetuses.On d 35 of gestation,Hu ewes(n=32)carrying twin fetuses were randomized into 4 groups(8 ewes and 16 fetuses in each group),where diets were as follows:100％of nutrient requirements recommended by National Research Council(NRC,2007)(CON);50％of nutrient requirements recommended by NRC(2007)(RES);RES+RP-Arg(20 g/d),(RES+ARG);and RES+NCG(5 g/d),(RES+NCG).On d 110 of gestation,both fetal and maternal tissues were collected and weighed.Compared with RES,solute carrier family 1,member 5(SLC1A5)was upregulated(P＜0.05)within fetal jejunum,duodenum and ileum when supplementing NCG and RP-Arg.Relative to RES,RP-Arg or NCG supplementation to RES resulted in upregulation(P＜0.05)of peptide transporter 1 protein abundance within the fetal ileum.NCG or RP-Arg supple-mentation to RES also upregulated phosphorylated mechanistic target of rapamycin(pmTOR)-to-mTOR ratio in the fetal ileum induced by IUGR(P＜0.05).As a result,during IUGR,supplementation of Arg or NCG affected intestinal AA profiles in the fetus in part through controlling mTOR signal transduction as well as AA and peptide transport.Future studies should be conducted to understand the role(if any)of the placenta on the improvement of growth and AA profiles independent of the fetal intestine.This would help demonstrate the relative contribution of intestinal uptake in fetal life.

Hao Zhang;Xiaoyun Liu;Yi Zheng;Ying Zhang;Juan J.Loor;Hongrong Wang;Mengzhi Wang

State Key Laboratory of Sheep Genetic Improvement and Healthy Production,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China;Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition,College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety,The Ministry of Education of China,Yangzhou University,Yangzhou 225009,China;Department of Animal Sciences and Division of Nutritional Sciences,University of Illinois,Urbana,IL 61801,USA

动物营养（英文）

[1]Hao Zhang;Xiaoyun Liu;Yi Zheng;Ying Zhang;Juan J.Loor;Hongrong Wang;Mengzhi Wang-.Dietary N-carbamylglutamate or L-arginine improves fetal intestinal amino acid profiles during intrauterine growth restriction in undernourished ewes)[J].动物营养（英文）,2022(01):341-349

carbamylglutamate,undernourished,ewes,postpartal,prepartum,RES+RP,RES+ARG,RES+NCG

Dietary,arginine,improves,fetal,intestinal,amino,acid,profiles,during,intrauterine,growth,restriction,Our,previous,studies,demonstrated,that,prenatal,utero,impairs,postnatal,function,Thus,improving,absorption,capacity,by,manipulating,maternal,importance,This,work,was,conducted,determine,whether,rumen,protected,Arg,increased,AA,retardation,IUGR,fetuses,On,gestation,Hu,carrying,twin,were,randomized,into,groups,each,where,diets,follows,nutrient,requirements,recommended,National,Research,Council,NRC,CON,both,tissues,collected,weighed,Compared,solute,carrier,family,member,SLC1A5,upregulated,within,jejunum,duodenum,ileum,when,supplementing,Relative,supplementation,resulted,upregulation,peptide,transporter,protein,abundance,also,phosphorylated,mechanistic,target,rapamycin,pmTOR,ratio,induced,affected,through,controlling,signal,transduction,well,Future,should,understand,role,any,placenta,improvement,independent,intestine,would,help,relative,contribution,uptake,life

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