Ferulic acid(FA)and vanillic acid(VA)are considered as major phenolic metabolites of cyanidin 3-glucoside,a polyphenol that widely exists in plants that possess a protective effect against oxidative stress and inflammation in our previous study.This study aimed to investigate the effect of FA and VA on inflammation,gut barrier function,and growth performance in a weaned piglet model challenged with lipopolysaccharide(LPS).Thirty-six piglets(PIC 337×C48,28 d of age)were randomly allocated into 3 treatments with 6 replicate pens(2 piglets per pen).They were fed with a basal diet or a diet containing 4,000 mg/kg of FA or VA.Dietary supplementation of VA significantly increased average daily gain(ADG)(P＜0.05).Both FA and VA decreased serum levels of thiobarbituric acid reactive substances(TBARS),interlukin(IL)-1β,IL-2,IL-6,and tumor necrosis factor(TNF)-α(P＜0.05),and enhanced the expression of tight junction protein oclaudin(P＜0.05).Analysis of gut microbiota indicated that both FA and VA increased the Firmicutes/Bacteroidetes ratio alongside reducing the relative abundance of the Pre-votellaceae family including Prevotella 9 and Prevotella 2 genera,but enriched the Lachoiraceaea family including the Lachnospiraceae FCS020 group(P＜0.05).Moreover,VA reduced the relative abundance of Prevotella 7 and Prevotella 1 but enriched Lachnospira,Eubacterium eligens group,and Eubacterium xylanophilum group(P＜0.05),while FA showed a limited effect on these genera.The results demon-strated that both VA and FA could alleviate inflammation and oxidative stress,but only VA has a sig-nificant positive effect on the growth performance of LPS-challenged piglets potentially through modulating gut microbiota.

Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients,College of Animal Science and Technology,Hunan Agricultural University,Changsha,410128,China;Beijing China-Agri HongKe Bio-Technology Co.,Ltd.,Beijing,102206,China;State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing,100193,China

[1]Ruizhi Hu;Shusong Wu;Baizhen Li;Jijun Tan;Jiahao Yan;Ying Wang;Zhiyi Tang;Ming Liu;Chenxing Fu;Hongfu Zhang;Jianhua He-.Dietary ferulic acid and vanillic acid on inflammation,gut barrier function and growth performance in lipopolysaccharide-challenged piglets)[J].动物营养（英文）,2022(01):144-152

oclaudin,votellaceae,Lachoiraceaea,FCS020,eligens

Dietary,ferulic,acid,vanillic,inflammation,gut,barrier,function,growth,performance,lipopolysaccharide,challenged,piglets,Ferulic,FA,VA,are,considered,major,phenolic,metabolites,cyanidin,glucoside,polyphenol,that,widely,exists,plants,possess,protective,effect,against,oxidative,stress,our,previous,study,This,aimed,investigate,weaned,model,LPS,Thirty,six,PIC,C48,were,randomly,allocated,into,treatments,replicate,pens,They,fed,basal,diet,containing,supplementation,significantly,increased,average,daily,ADG,Both,decreased,serum,levels,thiobarbituric,reactive,substances,TBARS,interlukin,tumor,necrosis,enhanced,expression,tight,junction,protein,Analysis,microbiota,indicated,both,Firmicutes,Bacteroidetes,ratio,alongside,reducing,relative,abundance,family,including,Prevotella,genera,but,enriched,Lachnospiraceae,group,Moreover,reduced,Eubacterium,xylanophilum,while,showed,limited,these,results,demon,strated,could,alleviate,only,has,positive,potentially,through,modulating

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