Ferrous glycinate(Fe-Gly)has been increasingly used as iron fortification in the diets of weaned piglets and broilers,but the effect of Fe-Gly on intestinal barrier function in meat ducks has not been well defined.This study therefore investigated the effect of Fe-Gly on apparent nutrient utilization,hema-tological indices,intestinal morphological parameters,intestinal barrier function and microbial composition in meat ducks.A total of 672 one-day-old Cherry Valley ducks were randomly divided into 6 treatments(8 replicates for each treatment and 14 ducks for each replicate)and fed diets with 0(con-trol),30,60,90 and 120 mg/kg Fe-Gly or 120 mg/kg FeSO4 for 35 d.The results showed that diets supplemented with Fe-Gly significantly increased average daily gain(ADG),average daily feed intake(ADFI),hematocrit(HCT),mean cell volume(MCV),the apparent utilization of dry matter(DM)and metabolizable energy(ME),villus height(VH)and villus height-to-crypt depth ratio(V:C)(P＜0.05).Fe-Gly also significantly up-regulated barrier-related genes including zonula occludens-1(ZO-1),zonula occludens-2(ZO-2),mucin 2(MUC2)and lysozyme(LYZ)(P＜0.05),and down-regulated the mRNA expression of claudin-2(CLDN2)and occludin(OCLN)in the jejunum(P＜0.05).The 16S rRNA sequence analysis indicated that the diet with Fe-Gly had a higher relative abundance of Intestinimonas and Romboutsia(P＜0.05),which have an ability to produce short chain fatty acids(SCFAs),especially butyric acid.It also decreased the relative abundance of pathobiont,including Megamonas,Eubacter-ium_coprostanoligenes_group and Plebeius(P＜0.05).Additionally,diets supplemented with 120 mg/kg Fe-Gly significantly increased the apparent utilization of DM and ME(P＜0.05)and decreased the relative abundance of Megamonas_unclassified and Bacteroides_unclassified compared with those fed 120 mg/kg FeSO4(P＜0.05).These results revealed that diets supplemented with Fe-Gly exerted a potent beneficial effect on physical,chemical,immune and microbial barriers,thereby improving the integrity of the intestinal structure,promoting the digestion and absorption of nutrients to a certain extent,and ultimately elevating the growth performance of ducks.

Haihua Yu;Yueqin Xie;Bing Wu;Hua Zhao;Xiaoling Chen;Gang Tian;Guangmang Liu;Jingyi Cai;Gang Jia

Institute of Animal Nutrition,Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Sichuan Agricultural University,Chengdu,Sichuan,611130,China;Chelota Group,Guanghan,618300,China

动物营养（英文）

[1]Haihua Yu;Yueqin Xie;Bing Wu;Hua Zhao;Xiaoling Chen;Gang Tian;Guangmang Liu;Jingyi Cai;Gang Jia-.Dietary supplementation of ferrous glycinate improves intestinal barrier function by modulating microbiota composition in Cherry Valley ducks)[J].动物营养（英文）,2022(04):264-275

glycinate,tological,metabolizable,CLDN2,pathobiont,Eubacter,Plebeius

Dietary,supplementation,ferrous,improves,intestinal,function,modulating,microbiota,composition,Cherry,Valley,ducks,Ferrous,Gly,has,been,increasingly,used,iron,fortification,diets,weaned,piglets,broilers,effect,meat,not,well,defined,This,study,therefore,investigated,apparent,utilization,indices,morphological,parameters,microbial,total,one,day,old,were,randomly,divided,into,treatments,replicates,each,fed,con,trol,FeSO4,results,showed,that,supplemented,significantly,increased,average,daily,gain,ADG,feed,intake,ADFI,hematocrit,HCT,mean,cell,volume,MCV,dry,matter,DM,energy,ME,villus,height,VH,crypt,depth,ratio,also,regulated,related,including,zonula,occludens,ZO,mucin,MUC2,lysozyme,LYZ,down,expression,claudin,occludin,OCLN,jejunum,16S,rRNA,sequence,analysis,indicated,had,higher,relative,abundance,Intestinimonas,Romboutsia,which,have,ability,produce,short,chain,fatty,acids,SCFAs,especially,butyric,It,decreased,Megamonas,ium,coprostanoligenes,group,Additionally,unclassified,Bacteroides,compared,those,These,revealed,exerted,potent,beneficial,physical,chemical,immune,barriers,thereby,improving,integrity,structure,promoting,digestion,absorption,nutrients,certain,extent,ultimately,elevating,growth,performance

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