Based on preparation of co-fermented defatted rice bran(DFRB)using Bacillus subtilis,Saccharomyces cerevisiae,Lactobacillus plantarum and phytase,the present study aimed to evaluate the effects of co-fermented DFRB on growth performance,antioxidant capacity,immune status,gut microbiota and permeability in finishing pigs.Ninety finishing pigs(85.30±0.97 kg)were randomly assigned to 3 treatments(3 replicates/treatment)with a basal diet(Ctrl),a basal diet supplemented with 10％unfer-mented DFRB(UFR),and a basal diet supplemented with 10％fermented DFRB(FR)for 30 d.Results revealed that the diet supplemented with FR notably(P＜0.05)improved the average daily gain(ADG),gain to feed ratio(G:F)and the digestibility of crude protein,amino acids and dietary fiber of finishing pigs compared with UFR.Additionally,FR supplementation significantly(P＜0.05)increased total antioxidant capacity,the activities of superoxide dismutase and catalase,and decreased the content of malonaldehyde in serum.Furthermore,FR remarkably(P＜0.05)increased serum levels of IgG,anti-inflammatory cytokines(IL-22 and IL-23)and reduced pro-inflammatory cytokines(TNF-α,IL-1β and INF-γ).The decrease of serum diamine oxidase activity and serum D-lactate content in the FR group(P＜0.05)suggested an improvement in intestinal permeability.Supplementation of FR also elevated the content of acetate and butyrate in feces(P＜0.05).Moreover,FR enhanced gut microbial richness and the abundance of fiber-degrading bacteria such as Clostridium butyricum and Lactobacillus amylovorus.Cor-relation analyses indicated dietary fiber in FR was associated with improvements in immune status,intestinal permeability and the level of butyrate-producing microbe C.butyricum,which was also verified by the in vitro fermentation analysis.These findings provided an experimental and theoretical basis for the application of fermented DFRB in finishing pigs.

Weifa Su;Zipeng Jiang;Cheng Wang;Yu Zhang;Tao Gong;Fengqin Wang;Mingliang Jin;Yizhen Wang;Zeqing Lu

Key Laboratory of Molecular Animal Nutrition,Ministry of Education,Zhejiang University,866 Yuhang Tang Road,Hangzhou,Zhejiang 310058,China;Key Laboratory of Animal Nutrition and Feed,Ministry of Agriculture,Zhejiang University,866 Yuhang Tang Road,Hangzhou,Zhejiang 310058,China;National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control,Zhejiang University,866 Yuhang Tang Road,Hangzhou,Zhejiang 310058,China;Key Laboratory of Animal Nutrition and Feed Science of Zhejiang Province,Zhejiang University,866 Yuhang Tang Road,Hangzhou,Zhejiang 310058,China;College of Animal Science,Institute of Feed Science,Zhejiang University,866 Yuhang Tang Road,Hangzhou,Zhejiang 310058,China

动物营养（英文）

[1]Weifa Su;Zipeng Jiang;Cheng Wang;Yu Zhang;Tao Gong;Fengqin Wang;Mingliang Jin;Yizhen Wang;Zeqing Lu-.Co-fermented defatted rice bran alters gut microbiota and improves growth performance,antioxidant capacity,immune status and intestinal permeability of finishing pigs)[J].动物营养（英文）,2022(04):413-424

DFRB,phytase,unfer,amylovorus

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