Objective:To evaluate the effect of Shilajit,a medicine of Ayurveda,on the serum changes in cytokines and adipokines caused by non-alcoholic fatty liver disease(NAFLD).Methods:After establishing fatty liver models by feeding a high-fat diet(HFD)for 12 weeks,35 Wistar male rats were randomly divided into 5 groups,including control(standard diet),Veh(HFD+vehicle),high-dose Shilajit[H-Sh,HFD+250 mg/(kg·d)Shilajit],low-dose Shilajit[L-Sh,HFD+150 mg/(kg·d)Shilajit],and pioglitazone[HFD+10 mg/(kg·d)pioglitazone]groups,7 rats in each group.After 2-week of gavage administration,serum levels of glucose,insulin,interleukin 1beta(IL-1 β),IL-6,IL-10,tumor necrosis factor-alpha(TNF-a),adiponectin,and resistin were measured,and insulin resistance index(HOMA-IR)was calculated.Results:After NAFLD induction,the serum level of IL-10 significantly increased and serum IL-1 β,TNF-a levels significantly decreased by injection of both doses of Shilajit and pioglitazone(P＜0.05).Increases in serum glucose level and homeostasis model of HOMA-IR were reduced by L-Sh and H-Sh treatment in NAFLD rats(P＜0.05).Both doses of Shilajit increased adiponectin and decreased serum resistin levels(P＜0.05).Conclusion:The probable protective role of Shilajit in NAFLD model rats may be via modulating the serum levels of IL-1 β,TNF-a,IL-10,adipokine and resistin,and reducing of HOMA-IR.

Baran Ghezelbash;Nader Shahrokhi;Mohammad Khaksari;Gholamreza Asadikaram;Maryam Shahrokhi;Sara Shirazpour

Physiology Research Center,Kerman University of Medical Sciences,Kerman(7616914115),Iran;Endocrinology,and Metabolism Research Center,Kerman University of Medical Sciences,Kerman(7616914115),Iran;Department of Biochemistry,School of Medicine,Kerman University of Medical Sciences,Kerman(7616914115),Iran;Department of Medical Science,School of Dentistry,Shiraz University of Medical Sciences,Shiraz(713414336),Iran;Department of Physiology and Pharmacology,Faculty of Medicine,Kerman University of Medical Sciences,Kerman(7616914115),Iran

中国结合医学杂志（英文版）

[1]Baran Ghezelbash;Nader Shahrokhi;Mohammad Khaksari;Gholamreza Asadikaram;Maryam Shahrokhi;Sara Shirazpour-.Protective Roles of Shilajit in Modulating Resistin,Adiponectin,and Cytokines in Rats with Non-alcoholic Fatty Liver Disease)[J].中国结合医学杂志（英文版）,2022(06):531-537

Shilajit,adipokines,HFD+vehicle,HFD+250,HFD+150,pioglitazone,HFD+10

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