Objective:The present study explored the mechanisms involved in intestinal lymphatic ligation in rats with severe acute pancreatitis(SAP).Methods:Male Sprague Dawley rats were randomly divided into 4 groups:saline group,saline+ligation group,SAP group,and SAP+ligation group.Rats in the SAP group were administered sodium taurocholate solution.Isolated mesenteric lymph duct ligation was administered to the saline+ligation and SAP+ligation groups.Endotoxin(ET),nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-1(IL-1),myeloperoxidase(MPO),and superoxide dismutase(SOD)were detected.Nuclear factor-KB(NF-κB)and intercellular cell adhesion molecule-1(ICAM-1)proteins were observed.The mRNA of inducible nitric oxide synthase(iNOS)and Toll-like receptor 4(TLR4)was detected by PCR.Results:Pathomorphological analysis showed that necrosis was present in the lung of rats in the SAP group,but only mild lesions in the SAP+ligation group.ET,NO,TNF-α,and IL-1 in the serum and lung tissue were significantly decreased and MPO was increased in the SAP+ligation group as compared with the SAP group.However,MPO was increased.The expression of NF-κB and ICAM-1,either iNOS or TLR4,was upregulated in the SAP group,but downregulated in the SAP+ligation group.Intestinal lymph duct ligation prevented ET translocation,the release of inflammation factors,and inflammation injury.Conclusion:The intestinal lymph duct ligation could alleviate SAP-induced pulmonary injury by suppressing NF-κB activation in rats.

Yuan-qi LIU;Lu-lu WANG;Li CHEN;Yu-xia XIONG

Department of Pharmacology,School of Pharmacy,Southwest Medical University,Luzhou 646000,China;Chengdu Vocational and Technical College,Chengdu,610000,China;Department of Pharmacy,the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou 646000,China

当代医学科学(英文)

[1]Yuan-qi LIU;Lu-lu WANG;Li CHEN;Yu-xia XIONG-.Influence of Intestinal Lymphatic Ligation on Pulmonary Injury in Rats with Severe Acute Pancreatitis)[J].当代医学科学(英文),2022(04):711-719

saline+ligation,SAP+ligation,Pathomorphological

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