OBJECTIVE:To investigate the protective effects and molecular mechanisms of Lizhong decoction(理中汤,LZD)against non-alcoholic fatty liver disease(NAFLD).METHODS:Male Wistar rats were fed a high-fat diet for four weeks to induce NAFLD,and were administered LZD by gavage for four weeks.Potential therapeutic targets for NAFLD were analyzed using network pharmacology.Liver pathology was evaluated using Oil Red O and hematoxylin-eosin staining.Furthermore,mitochondrial function,lipid metabolism,oxidative stress,and inflammatory response were examined.RESULTS:Rats with NAFLD exhibited high levels of hepatic damage and cholesterol deposition.Moreover,apoptosis was increased,superoxide dismutase and glutathione content were reduced,malondialdehyde content was increased,and the protein expression of inflammatory cytokines and p-c-Jun N-terminal kinase was increased.The LZD treatment ameliorated mitochondrial dysfunction,reduced liver damage,inhibited oxidative stress and inflammatory response,upregulated peroxisome proliferator-activated receptor(PPAR)-γ expression,and suppressed dipeptidyl peptidase 4(DPP4)expression in the liver.CONCLUSION:It was found that LZD alleviates NAFLD by activating PPAR-γ and inhibiting DPP4.

YANG Jiayao;TAO Dongqing;MA Wei;LIU Song;LIAO Yan;SHU Lei;ZHANG Shu;LI Chenyu;DU Nianlong

Department of Gastroenterology,Wuhan Integrated TCM and Western Medicine Hospital,Wuhan 430022,China;Department of Endocrinology,The Third People's Hospital of Hubei Province,Wuhan 430033,China;Department of Center Laboratory,Wuhan Integrated TCM and Western Medicine Hospital,Wuhan 430022,China;Hubei University of Traditional Chinese Medicine,Wuhan 430065,China

中医杂志（英文版）

[1]YANG Jiayao;TAO Dongqing;MA Wei;LIU Song;LIAO Yan;SHU Lei;ZHANG Shu;LI Chenyu;DU Nianlong-.Protective effects and mechanisms of Lizhong decoction(理中汤)against non-alcoholic fatty liver disease in a rat model)[J].中医杂志（英文版）,2022(05):773-780

Lizhong

Protective,effects,mechanisms,decoction,理中汤,against,alcoholic,fatty,liver,disease,model,OBJECTIVE,To,investigate,protective,molecular,LZD,NAFLD,METHODS,Male,Wistar,rats,were,fed,high,diet,four,weeks,induce,administered,by,gavage,Potential,therapeutic,targets,analyzed,using,network,pharmacology,Liver,pathology,was,evaluated,Oil,Red,hematoxylin,eosin,staining,Furthermore,mitochondrial,lipid,metabolism,oxidative,stress,inflammatory,response,examined,RESULTS,Rats,exhibited,levels,hepatic,damage,cholesterol,deposition,Moreover,apoptosis,increased,superoxide,dismutase,glutathione,content,reduced,malondialdehyde,protein,expression,cytokines,Jun,terminal,kinase,treatment,ameliorated,dysfunction,inhibited,upregulated,peroxisome,proliferator,activated,receptor,PPAR,suppressed,dipeptidyl,peptidase,DPP4,CONCLUSION,It,found,that,alleviates,activating,inhibiting

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