OBJECTIVE:To evaluate the therapeutic effectiveness of salidroside(Sal)and pyrrolidine dithiocarbamate(PDTC)against severe acute pancreatitis(SAP)in a rat model.METHODS:Rat models of SAP were established by retrograde infusion of sodium taurocholate solution.SAP rats were randomly divided into 6 groups:SAP 3 h group,SAP 24 h group,low-dose Sal treatment group(Sal L+S),middle-dose Sal treatment group(Sal M+S),high-dose Sal treatment group(Sal H+S)and PDTC treatment group(PDTC+S).The serum amylase,tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1β)and interleukin-10(IL-10)levels were determined by optical turbidimetry and enzyme-linked immunosorbent assay.The expression of Beclin-1,microtubule-associated protein light chain 3Ⅱ(LC3 Ⅱ),lysosome associated membrane protein 2(LAMP2),interleukin-1 receptor associated kinase 1(IRAK1),inhibitor α of nuclear transcription factor-KB(IκBα),nuclear transcription factor-KB 65(p65)in the pancreas tissues were detected by quantitative real-time polymerase chain reaction and Western blot,while the p-IκBα and p-p65 levels were detected by Western blot.Pathological changes of the pancreas and all the other indexes were observed at 3 and 24 h after operation.RESULTS:The serum IL-10 level,IκBα and LAMP2 levels in Sal M+S,Sal H+S and PDTC+S groups were higher than those in SAP 24 h group,while all the other indexes in these three groups were all lower significantly than those in SAP 24 h group.There was no significant difference in all indexes between Sal H+S and PDTC+S groups.CONCLUSION:High-dose Sal has an effectively therapeutic effect on SAP in rats,which was similar to PDTC.

QIAN Jing;WANG Xiaohong;WEI Benzhong;ZHOU Guoxiong;ZHU Shunxing;LIU Chun

Department of General Surgery,Yizheng Hospital of Nanjing Drum Tower Hospital Group,Yizheng 211900,China;Department of Gastroenterology,Yizheng Hospital of Nanjing Drum Tower Hospital Group,Yizheng 211900,China;Department of Anesthesiology,Yizheng Hospital of Nanjing Drum Tower Hospital Group,Yizheng 211900,China;Department of Gastroenterology,Affiliated Hospital of Nantong University,Nantong 226001,China;Department of Laboratory Animal Center,Nantong University,Nantong 226001,China

中医杂志（英文版）

[1]QIAN Jing;WANG Xiaohong;WEI Benzhong;ZHOU Guoxiong;ZHU Shunxing;LIU Chun-.Therapeutic effects of salidroside vs pyrrolidine dithiocarbamate against severe acute pancreatitis in rats)[J].中医杂志（英文版）,2022(01):49-57

L+S,M+S,H+S,PDTC+S,turbidimetry

Therapeutic,effects,salidroside,pyrrolidine,dithiocarbamate,against,severe,acute,pancreatitis,rats,OBJECTIVE,To,evaluate,therapeutic,effectiveness,Sal,SAP,METHODS,Rat,models,were,established,by,retrograde,infusion,sodium,taurocholate,solution,randomly,divided,into,groups,dose,treatment,middle,serum,amylase,tumor,necrosis,interleukin,levels,determined,optical,enzyme,linked,immunosorbent,assay,expression,Beclin,microtubule,associated,protein,light,chain,LC3,lysosome,membrane,LAMP2,receptor,kinase,IRAK1,inhibitor,nuclear,transcription,KB,p65,pancreas,tissues,detected,quantitative,real,polymerase,reaction,blot,while,Pathological,changes,all,other,indexes,observed,after,operation,RESULTS,higher,than,those,these,three,lower,significantly,There,was,difference,between,CONCLUSION,High,has,effectively,which,similar

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