OBJECTIVE:To investigate the effect of manipulation treatment on knee osteoarthritis rats and the effect on Rho-associated protein kinase(ROCK)/LIM-kinase1(LIMK1)/Cofilin signaling pathway.METHOD:Fifty Specific pathogen Free Sprague-Dawley rats were randomly divided into five groups(n=8 each):blank group,model group,manipulation group,celecoxib group,and manipulation combined with celecoxib group(MC group).The osteoarthritis model was established by injecting 0.2 mL 4％papain into the articular disc of the rats.After successfully establishing the model,we treated the manipulation group with pushing manipulation using one-finger-meditation to the Neixiyan(EX-LE4),Waixiyan(EX-LE5),Xuehai(SP10),Liangqiu(ST34),and Zusanli(ST36)acupoints for 10 min each time.Also,the celecoxib group was gavaged with 24 mg·kg-1·d-1 celecoxib,while the MC group was treated using both of these two methods.After four weeks,the cartilage of the right femur was removed for hematoxylin-eosin staining of the cartilage tissue.The expressions of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum were observed using the enzyme-linked immunosorbent assay.Besides,we detected the expressions of ROCK,LIMK1,Phospho-LIM-kinase1(Phospho-LIMK1),Cofilin,and Phospho-Cofilin by Western blot.RESULTS:Compared to the model group,the manipulation group,celecoxib group,and MC group all exhibited superior results concerning pathological morphologic changes of cartilage,as observed by hematoxylin-eosin staining and calculated using the Mankin score.Besides,in contrast to the blank group,the model group exhibited elevated serum levels of IL-1 β and TNF-α(P＜0.01),while the expression of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage were all higher(P＜0.01).Also,the serum levels of IL-1 βand TNF-α in each treatment group were lower(P＜0.01)than in the model group.Moreover,there were lower expressions of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage in the manipulation group and the MC group(P＜0.01).Compared with the model group,the expression of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage in the celecoxib group were not statistically different(P＞0.05).CONCLUSION:In this study,we established that manipulation has a better curative effect than celecoxib.Manipulation inhibits the development of cytoskeleton damage in cartilage and slows articular degeneration by regulating the expression of related proteins in the cytoskeletal signaling pathway.

GUO Xiao;YANG Yunhao;LIAO Dongmei;PANG Fang;YANG Zhixue;ZHU Zhengwei;LUO Ao;TANG Chenglin

College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing 400010,China Beibei Traditional Chinese Medicine Hospital,Chongqing 400700,China

中医杂志（英文版）

[1]GUO Xiao;YANG Yunhao;LIAO Dongmei;PANG Fang;YANG Zhixue;ZHU Zhengwei;LUO Ao;TANG Chenglin-.Effect of manipulation on cartilage in rats with knee osteoarthritis based on the Rho-associated protein kinase/LIM kinase 1/Cofilin signaling pathways)[J].中医杂志（英文版）,2022(02):194-199

Neixiyan,LE4,Waixiyan,LE5,Xuehai,Liangqiu

Effect,manipulation,cartilage,rats,knee,osteoarthritis,Rho,associated,Cofilin,signaling,pathways,OBJECTIVE,To,investigate,effect,treatment,ROCK,kinase1,LIMK1,METHOD,Fifty,Specific,pathogen,Free,Sprague,Dawley,were,randomly,divided,into,five,groups,each,blank,model,celecoxib,combined,MC,was,established,by,injecting,papain,articular,disc,After,successfully,establishing,treated,pushing,using,one,finger,meditation,EX,SP10,ST34,Zusanli,ST36,acupoints,Also,gavaged,while,both,these,two,methods,four,weeks,right,femur,removed,hematoxylin,eosin,staining,tissue,expressions,interleukin,tumor,necrosis,serum,observed,enzyme,linked,immunosorbent,assay,Besides,detected,Phospho,blot,RESULTS,Compared,exhibited,superior,results,concerning,pathological,morphologic,changes,calculated,Mankin,score,contrast,elevated,levels,higher,lower,than,Moreover,there,not,statistically,different,CONCLUSION,In,this,study,that,has,better,curative,Manipulation,inhibits,development,cytoskeleton,damage,slows,degeneration,regulating,related,proteins,cytoskeletal

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