Helichrysetin(HEL),a chalcone isolated from Alpinia katsumadai Hayata,has an antitumor activity in human lung and cervical cancers.However,the inhibitory effect and underlying mechanism of HEL in gastric cancer have not been elucidated.Here,HEL significantly inhibited the growth of gastric cancer MGC803 cells in vitro and in vivo.HEL decreased expression and transcriptional regulatory activity of c-Myc and mRNA expression of c-Myc target genes.HEL enhanced mitochondrial oxidative phosphorylation(OXPHOS)and reduced glycolysis as evidenced by increased mitochondrial adenosine triphosphate(ATP)production and excessive reactive oxygen species(ROS)accumulation,and decreased the pPDHA1/PDHA1 ratio and Glyco-ATP production.Pyruvate enhanced OXPHOS after HEL treatment.c-Myc overexpression abolished HEL-induced inhibition of cell viability,glycolysis,and protein expression of PDHK1 and LDHA.PDHK1 overexpression also counteracted inhibitory effect of HEL on cell viability.Conversely,c-Myc siRNA decreased cell viability,glycolysis,and PDHK1 expression.NAC rescued the decrease in viability of HEL-treated cells.Additionally,HEL inhibited the overactivated mTOR/p70S6K pathway in vitro and in vivo.HEL-induced cell viability inhibition was counteracted by an mTOR agonist.mTOR inhibitor also decreased cell viability.Similar results were obtained in SGC7901 cells.HEL repressed lactate production and efflux in MGC803 cells.These results revealed that HEL inhibits gastric cancer growth by targeting mTOR/p70S6K/c-Myc/PDHK1-mediated energy metabolism reprogramming in cancer cells.Therefore,HEL may be a potential agent for gastric cancer treatment by modulating cancer energy metabolism reprogramming.

Ping Wang;Jin-mei Jin;Xiao-hui Liang;Ming-zhu Yu;Chun Yang;Fei Huang;Hui Wu;Bei-bei Zhang;Xiao-yan Fei;Zheng-tao Wang;Ren Xu;Hai-lian Shi;Xiao-jun Wu

Shanghai Key Laboratory of Compound Chinese Medicines,The Ministry of Education(MOE)Key Laboratory for Standardization of Chinese Medicines,The SATCM Key Laboratory for New Resources&Quality Evaluation of Chinese Medicine,Research Center of Shanghai Traditional Chinese Medicine Standardization,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Markey Cancer Center,Department of Pharmacology and Nutritional Sciences,University of Kentucky College of Medicine,Lexington,KY,USA

中国药理学报（英文版）

[1]Ping Wang;Jin-mei Jin;Xiao-hui Liang;Ming-zhu Yu;Chun Yang;Fei Huang;Hui Wu;Bei-bei Zhang;Xiao-yan Fei;Zheng-tao Wang;Ren Xu;Hai-lian Shi;Xiao-jun Wu-.Helichrysetin inhibits gastric cancer growth by targeting c-Myc/PDHK1 axis-mediated energy metabolism reprogramming)[J].中国药理学报（英文版）,2022(06):1581-1593

Helichrysetin,PDHK1,katsumadai,pPDHA1,overactivated

inhibits,gastric,growth,by,targeting,Myc,axis,mediated,energy,metabolism,reprogramming,HEL,chalcone,isolated,from,Alpinia,Hayata,has,antitumor,activity,human,lung,cervical,cancers,However,inhibitory,effect,underlying,mechanism,have,not,been,elucidated,Here,significantly,inhibited,MGC803,cells,vitro,vivo,decreased,transcriptional,regulatory,genes,enhanced,mitochondrial,oxidative,phosphorylation,OXPHOS,reduced,glycolysis,evidenced,increased,adenosine,triphosphate,ATP,production,excessive,reactive,oxygen,species,ROS,accumulation,ratio,Glyco,Pyruvate,after,treatment,overexpression,abolished,induced,inhibition,viability,protein,LDHA,also,counteracted,Conversely,siRNA,NAC,rescued,treated,Additionally,mTOR,p70S6K,pathway,was,agonist,Similar,results,were,obtained,SGC7901,repressed,lactate,efflux,These,revealed,that,Therefore,may,potential,agent,modulating

0.436632