OBJECTIVE:To elucidate the potential molecular mechanism by which Fuzheng Kang'ai decoction(扶正抗癌方,FZKA)inhibits proliferation,migration,and invasion of lung cancer cells.METHODS:Varying FZKA concentrations were used to manage lung cancer cells(A549 and PC9).We employed:cell counting kit-8(CCK-8)and plate clone for-mation assays to examine the cell viability;flow cytometry(FCM)to analyze the cycle arrest;transwell and wound-healing assays to assess the cell invasion and migration,respectively.Further,a quantitative real-time polymerase chain reaction(qRT-PCR)assay was adopted to evaluate the miR-21-5p expression.For protein expression analysis,we employed the Western blot technique.Recombinant miR-21-5p overexpression adenovirus vector harboring GFP was constructed and transfected into A549 and PC9,after which we explored the effect of FZKA on miR-21-5p overexpression.RESULTS:Notably,treatment with FZKA inhibited viability,clone-formation ability,invasion,and migration of lung cancer cells.Mechanistically,FZKA markedly suppressed miR-21-5p expression but elevated the human phosphatase and tensin homology deleted on chromosome ten(PTEN)protein level in both A549 and PC9 cells.Over-expression of miR-21-5p lowered PTEN protein expression.Besides,overexpressed miR-21-5p levels with adenovirus antagonized FZKA-upregulated PTEN protein expression.CONCLUSION:The present study demonstrates how FZKA modulates cell biological behaviors,for instance,it impedes the proliferation by upregulating PTEN expression with miR-21-5p as the target.These findings unveil the potential novel molecular mechanisms from the microRNA aspect by which FZKA suppresses the growth of human lung cancer cells.

LI Jinhua;LIU Chunping;ZHAO Yueyang;WU Wanyin;SUN Pengtao;LI Longmei;YANG Xiaobing;ZHOU Yushu

Department of Ultrasound,Guangdong Provincial Hospital of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;Department of Ultrasound,Institute of Ultrasound in Musculoskeletal Sports Medicine,Guangdong Second Provincial General Hospital,Guangzhou 510317,China;State Key Laboratory of Dampness Syndrome of Chinese Medicine,Guangdong Provincial Hospital of Chinese Medicine,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;Department of Hematology,Guangdong Provincial Hospital of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;Department of Oncology,Clinical and Basic Research Team of TCM Prevention and Treatment of NSCLC,Guangdong Provincial Hospital of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China

中医杂志（英文版）

[1]LI Jinhua;LIU Chunping;ZHAO Yueyang;WU Wanyin;SUN Pengtao;LI Longmei;YANG Xiaobing;ZHOU Yushu-.Fuzheng Kang'ai decoction(扶正抗癌方)inhibits cell proliferation,migration and invasion by modulating mir-21-5p/human phosphatase and tensin homology deleted on chromosome ten in lung cancer cells)[J].中医杂志（英文版）,2022(03):344-352

FZKA

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