典型文献
Visualization of the process:selenocysteine activates GPX4 in ferroptosis based on a nano-fluorescent probe
文献摘要：
Developing accurate methods for visualizing the expressions of proteins during the life process is a hotspot in the biochemical research field.Glutathione peroxidase 4(GPX4),one type of selenoproteins,was previously identified as a significant fer-roptosis-regulated protein.Therefore,a novel approach was established to visualize the activation process of selenocysteine(Sec)toward GPX4 via an Au-Se bonded fluorescent nanoprobe and assisted by western blot analysis.The Au-Se nanoprobe was constructed by selenol-modified peptides for monitoring the concentration changes of Sec.The fluorescence intensity of Sec in confocal fluorescence imaging positively correlates with GPX4's activation level,proved by western blot analysis in living cells.This study provides an intuitional and visual strategy for predicting the functional expression of GPX4 during the ferroptosis process and offers new directions for the future investigation of proteins'functional expressions.
文献关键词：
中图分类号：
作者姓名：
Dongrui Luan;Wenfei Guo;Xiaonan Gao;Kehua Xu;Bo Tang
作者机构：
College of Chemistry,Chemical Engineering and Materials Science,Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong,Key Laboratory of Molecular and Nano Probes,Ministry of Education,Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals,Shandong Normal University,Jinan 250014,China;Department of Clinical Laboratory,The Second Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250033,China
文献出处：
引用格式：
[1]Dongrui Luan;Wenfei Guo;Xiaonan Gao;Kehua Xu;Bo Tang-.Visualization of the process:selenocysteine activates GPX4 in ferroptosis based on a nano-fluorescent probe)[J].中国科学:化学（英文版）,2022(07):1286-1290
A类：
selenocysteine,selenol,intuitional
B类：
Visualization,process,activates,GPX4,ferroptosis,fluorescent,Developing,accurate,methods,visualizing,expressions,during,life,hotspot,biochemical,research,field,Glutathione,peroxidase,type,selenoproteins,was,previously,identified,significant,regulated,Therefore,novel,approach,established,visualize,activation,Sec,toward,via,Au,bonded,nanoprobe,assisted,by,western,blot,analysis,constructed,modified,peptides,monitoring,concentration,changes,fluorescence,intensity,confocal,imaging,positively,correlates,level,proved,living,cells,This,study,provides,strategy,predicting,functional,offers,new,directions,future,investigation
AB值：
0.524142
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