典型文献
Amplified visualization and function exploration of exosomal protein-specific glycosylation using hybridization chain reaction from non-functional epitope
文献摘要：
Exosomal glycoproteins play significant roles in many physiological and pathological procedures.However,the current methods for studying exosomal glycoproteins have low sensitivity or can affect exosomal biological function.Herein,we developed a proximity dual-tagging strategy using an induced hybridization chain reaction(HCR)from the target's non-functional epitope for amplified visualization and functional exploration of exosomal protein-specific glycosylation.This strategy leverages dual-tagging based on the aptamer with little influence on target function and metabolic glycan labelling,and the rigid product and high sensitivity of HCR.The method improves the signal of visualizing exosomal PD-L1(exoPD-L1)by 7.7-fold compared with the signal without HCR amplification without affecting the natural exoPD-Ll/PD-1 interaction.As a result,we verified that the interaction between exoPD-Ll and PD-1 positive cells is positively correlated to the glycosylation level of exoPD-Ll.Overall,we have developed a sensitive method with little functional influence to visualize exosomal protein-specific glycosylation in situ,offering a powerful tool for studying the biological implications of exosomal glycoproteins.
文献关键词：
中图分类号：
作者姓名：
Siyin Kang;Lin Zhu;Wencheng Wang;Yinzhu Lu;Zhenlong You;Chi Zhang;Yuanfeng Xu;Chaoyong Yang;Yanling Song
作者机构：
The MOE Key Laboratory of Spectrochemical Analysis&Instrumentation,the Key Laboratory of Chemical Biology of Fujian Province,State Key Laboratory of Physical Chemistry of Solid Surfaces,Department of Chemical Biology,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;Institute of Molecular Medicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China
文献出处：
引用格式：
[1]Siyin Kang;Lin Zhu;Wencheng Wang;Yinzhu Lu;Zhenlong You;Chi Zhang;Yuanfeng Xu;Chaoyong Yang;Yanling Song-.Amplified visualization and function exploration of exosomal protein-specific glycosylation using hybridization chain reaction from non-functional epitope)[J].中国科学:化学（英文版）,2022(06):1204-1211
A类：
exoPD
B类：
Amplified,visualization,exploration,exosomal,specific,glycosylation,using,hybridization,chain,reaction,from,functional,epitope,Exosomal,glycoproteins,play,significant,roles,many,physiological,pathological,procedures,However,current,methods,studying,have,low,sensitivity,biological,Herein,developed,proximity,dual,tagging,strategy,induced,HCR,target,amplified,This,leverages,aptamer,little,influence,metabolic,glycan,labelling,rigid,product,high,improves,signal,visualizing,L1,by,fold,compared,without,amplification,affecting,natural,Ll,interaction,result,verified,that,between,cells,positively,correlated,level,Overall,sensitive,visualize,situ,offering,powerful,tool,implications
AB值：
0.478529
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