典型文献
Characterization of two SARS-CoV-2 subgenomic RNA dynamics in severe COVID-19 patients
文献摘要：
Little is known about Subgenomic RNA(sgRNA)dynamics in patients with Coronavirus diseases 2019(COVID-19).We collected 147 throat swabs,74 gut swabs and 46 plasma samples from 117 COVID-19 patients recruited in the LOTUS China trial(ChiCTR2000029308)and compared E and orf7a sgRNA load in patients with different illness duration,outcome,and comorbidities.Both sgRNAs were detected in all the three types of samples,with longest duration of 25,13,and 17 days for E sgRNA,and 32,28,and 17 days for orf7a sgRNA in throat,gut,and plasma,respectively.A total of 95％(57/60)of patients had no E sgRNA detected after 10 days post treatment,though 86％of them were still E RNA positive.High correlation on titer was observed between sgRNA encoding E and orf7a gene.sgRNA showed similar variation in the standard care and Lopinavir-Ritonavir group.Patients with diabetes and heart diseases showed higher pharyngeal E sgRNA at the first day(P=0.016 and 0.013,respec-tively)but no difference at five days after treatment,compared with patients without such commodities.Patients with hypertension and cerebrovascular diseases showed no difference in the pharyngeal sgRNA levels at both one and five days after treatment,compared with patients without these two commodities.E sgRNA levels in the initial infection showed no correlation with the serum antibody against spike,nucleoprotein,and receptor binding domains at ten days later.sgRNA lasted a long period in COVID-19 patients and might have little effect on hu-moral response.
文献关键词：
中图分类号：
作者姓名：
Xiaohui Zou;Shengrui Mu;Yeming Wang;Li Guo;Lili Ren;Xiaoyan Deng;Haibo Li;Jiankang Zhao;Yulin Zhang;Hui Li;Binghuai Lu;Chaolin Huang;Bin Cao
作者机构：
Department of Pulmonary and Critical Care Medicine,Center of Respiratory Medicine,National Clinical Research Center for Respiratory Diseases,China-Japan Friendship Hospital,Beijing,100029,China;National Center for Respiratory Medicine,Beijing,100029,China;Institute of Respiratory Medicine,Chinese Academy of Medical Sciences,Beijing,100029,China;Department of Pulmonary and Critical Care Medicine,Capital Medical University,Beijing,100069,China;NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory,Institute of Pathogen Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,100176,China;Jin Yin-tan Hospital,Wuhan,430013,Hubei Province,China;Tsinghua University-Peking University Joint Center for Life Sciences,Beijing,100029,China
文献出处：
引用格式：
[1]Xiaohui Zou;Shengrui Mu;Yeming Wang;Li Guo;Lili Ren;Xiaoyan Deng;Haibo Li;Jiankang Zhao;Yulin Zhang;Hui Li;Binghuai Lu;Chaolin Huang;Bin Cao-.Characterization of two SARS-CoV-2 subgenomic RNA dynamics in severe COVID-19 patients)[J].中国病毒学,2022(01):30-37
A类：
subgenomic,Subgenomic,LOTUS,ChiCTR2000029308,orf7a,Lopinavir,Ritonavir
B类：
Characterization,two,SARS,CoV,dynamics,severe,patients,Little,known,about,Coronavirus,diseases,We,collected,throat,swabs,gut,plasma,samples,from,recruited,China,trial,compared,load,different,illness,duration,outcome,comorbidities,Both,sgRNAs,were,detected,all,three,types,longest,days,respectively,total,had,after,post,treatment,though,them,still,positive,High,correlation,titer,was,observed,between,encoding,gene,showed,similar,variation,standard,care,group,Patients,diabetes,heart,higher,pharyngeal,first,but,difference,five,without,such,commodities,hypertension,cerebrovascular,levels,both,one,these,initial,infection,serum,antibody,against,spike,nucleoprotein,receptor,binding,domains,later,lasted,period,might,have,little,effect,hu,moral,response
AB值：
0.47165
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