OBJECTIVE:To evaluate the effects of the Wenshen Jianpi recipe(温肾健脾方,WJR)on immune reconstruction and natural killer(NK)cells in immunological non-responders(INRs)of people living with human immunodeficiency virus(HIV)(PLWH)and propose new therapeutic strategies for HIV.METHODS:Based on Traditional Chinese Medicine treatment principle"invigorating Qi and warming Yang in the spleen and kidneys",WJR combined with antire-troviral therapy(ART)therapy was performed in a randomized,double-blind,placebo-controlled study of 60 patients with non-responders.The randomized process was executed by the Clinical Evaluation Center of China Academy of Chinese Medical Sciences.Sixty patients who met the inclusion criteria obtained random numbers(that is the drug number)was randomly divided into a treatment group and a placebo control group according to a 1:1 ratio.CD4+T cell counts and natural killer(NK)cells counts were evaluated at baseline and 12-week,24-week follow-ups.RESULTS:Four participants received random numbers and did not enter the group due to the patient's own reasons.A total of 56 patients were enrolled,including 28 in the treatment group and 28 in the control group.CD4+T cell counts in the treatment group were significantly increased at week 24(P=0.01＜0.05),which were significantly higher than those in the control group(P=0.01＜0.05).Although no significant differences were observed between two groups,the CD56briCD16-NK cell counts in the treatment group were significantly increased after duration.and CD56dimCD16+NK cell counts in the treatment group were significantly higher than those in the control group after 24 weeks of treatment(P=0.025＜0.05).As compared with the control group,the treatment group had significantly lower CD56negCD16+NK cell counts after 24 weeks of treatment(P=0.023＜0.05).CONCLUSIONS:WJR promotes the immune reconstruction of INRs and redistribution of NK cell subsets,notably decreasing CD56negCD16+NK cell counts in INRs.However,the redistribution of NK cell subsets is not beneficial for immune reconstruction in INRs.Further large-scale RCTs are required to evaluate the effect of WJR on immune recovery in INRs and decipher the underlying mechanism.

TAO Zhuang;WANG Jian;CHEN Xin;LI Yonghong;YAN Yuguang;ZHANG Ao;ZOU Wen;LIU Ying

Research Center of AIDS Treatment with Traditional Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;Department of Integrated Traditional Chinese and Wetern Medicine,Kunming Hospital of Traditional Chinese Medicine,Kunming 650000,China;Department of Infection,No.6 People's Hospital of Shenyang City,Shenyang 110006,China;University of Chinese Academy of SoCIal SCIences,Beijing,100102,China

中医杂志（英文版）

[1]TAO Zhuang;WANG Jian;CHEN Xin;LI Yonghong;YAN Yuguang;ZHANG Ao;ZOU Wen;LIU Ying-.Wenshen Jianpi recipe(温肾健脾方)induced immune reconstruction and redistribution of natural killer cell subsets in immunological non-responders of human immunodeficiency virus/acquired immune deficiency syndrome:a randomized controlled trial)[J].中医杂志（英文版）,2022(05):795-802

Wenshen,WJR,invigorating,antire,troviral,CD56briCD16,CD56dimCD16+NK,CD56negCD16+NK

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