典型文献
Phase separation of Ddx3xb helicase regulates maternal-to-zygotic transition in zebrafish
文献摘要：
Vertebrate embryogenesis involves a conserved and fundamental process,called the maternal-to-zygotic transition(MZT),which marks the switch from a maternal factors-dominated state to a zygotic factors-driven state.Yet the precise mechanism underlying MZT remains largely unknown.Here we report that the RNA helicase Ddx3xb in zebrafish undergoes liquid-liquid phase separation(LLPS)via its N-terminal intrinsically disordered region(IDR),and an increase in ATP content promotes the condensation of Ddx3xb during MZT.Mutant form of Ddx3xb losing LLPS ability fails to rescue the developmental defect of Ddx3xb-deficient embryos.Interestingly,the IDR of either FUS or hnRNPA1 can functionally replace the N-terminal IDR in Ddx3xb.Phase separation of Ddx3xb facilitates the unwinding of 5'UTR structures of maternal mRNAs to enhance their translation.Our study reveals an unprecedent mechanism whereby the Ddx3xb phase separation regulates MZT by promoting maternal mRNA translation.
文献关键词：
中图分类号：
作者姓名：
Boyang Shi;Jian Heng;Jia-Yi Zhou;Ying Yang;Wan-Ying Zhang;Magdalena J.Koziol;Yong-Liang Zhao;Pilong Li;Feng Liu;Yun-Gui Yang
作者机构：
CAS Key Laboratory of Genomic and Precision Medicine,Collaborative Innovation Center of Genetics and Development,College of Future Technology,Beijing Institute of Genomics, Chinese Academy of Sciences,Beijing,China;China National Center for Bioinformation,Beijing,China;Department of Pharmacy,The First Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan,China;State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,China;Institute of Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing,China;CAS Key Laboratory of Genomic and Precision Medicine,Collaborative Innovation Center of Genetics and Development,College of Future Technology,Beijing Institute of Genomics, Chinese Academy of Sciences,Beijing,China;University of Chinese Academy of Sciences,Beijing, China;Chinese Institute for Brain Research,Beijing,China;Beijing Advanced Innovation Center for Structural Biology,Beijing Frontier Research Center for Biological Structure, Tsinghua-Peking Joint Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing,China
文献出处：
引用格式：
[1]Boyang Shi;Jian Heng;Jia-Yi Zhou;Ying Yang;Wan-Ying Zhang;Magdalena J.Koziol;Yong-Liang Zhao;Pilong Li;Feng Liu;Yun-Gui Yang-.Phase separation of Ddx3xb helicase regulates maternal-to-zygotic transition in zebrafish)[J].细胞研究（英文版）,2022(08):715-728
A类：
Ddx3xb,Vertebrate,MZT,unwinding
B类：
Phase,separation,helicase,regulates,maternal,zygotic,transition,zebrafish,embryogenesis,involves,conserved,fundamental,process,called,which,marks,switch,from,factors,dominated,state,driven,Yet,precise,mechanism,underlying,remains,largely,unknown,Here,we,report,that,undergoes,liquid,phase,LLPS,via,its,terminal,intrinsically,disordered,region,IDR,increase,ATP,content,promotes,condensation,during,Mutant,form,losing,ability,fails,rescue,developmental,defect,deficient,embryos,Interestingly,either,FUS,hnRNPA1,can,functionally,replace,facilitates,UTR,structures,mRNAs,enhance,their,translation,Our,study,reveals,unprecedent,whereby,promoting
AB值：
0.542091
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
