典型文献
Upregulation of CENPM facilitates lung adenocarcinoma progression via PI3K/AKT/mTOR signaling pathway
文献摘要：
Centromere protein M(CENPM)is essential for chromosome separation during mitosis.However,its roles in lung adenocarcinoma(LUAD)progression and metastasis remain unknown.In this study,we aimed to explore the effects of CENPM on LUAD progression as well as the underlying mechanisms.We analyzed the expression of CENPM and its correlation with clinicopathological characteristics using GEO LUAD chip datasets and TCGA da-taset.We further investigated the impact of CENPM on LUAD in vitro and in vivo.In silico analysis and qRT-PCR revealed that CENPM is upregulated in LUAD compared with that in normal lung tissues.Via gain/loss-of-function assays,we further found that CENPM promotes the LUAD cell cycle,cell proliferation,migration and invasion,and inhibits cell apoptosis in vitro.The in vivo study showed that loss of CENPM inhibits the growth of A549 xenografts.Furthermore,we found that CENPM can promote the phosphorylation of mTOR rather than directly affect the mTOR content.Inhibition of mTOR activity abrogates the promoting effects of CENPM on cell cycle progression,cell proliferation,migration and invasion.Taken together,these results show that CENPM plays an important role in the growth and metastasis of LUAD and may be a promising therapeutic target in LUAD.
文献关键词：
中图分类号：
作者姓名：
Chao Liu;Yun Wang;Yuyang Dao;Shuting Wang;Fei Hou;Zhixian Yang;Pengjie Liu;Juan Lv;Ling Lv;Gaofeng Li;Youjun Zhou;Zhiyong Deng
作者机构：
Department of Nuclear Medicine,Tumor Hospital of Yunnan Province,the Third Affiliated Hospital of Kunming Medical College,Kunming 650118,China;Department of Thoracic Surgery,Tumor Hospital of Yunnan Province,the Third Affiliated Hospital of Kunming Medical College,Kunming 650118,China;Department of Nuclear Medicine,the Affiliated Yan'an Hospital of Kunming Medical College,Kunming 650118,China
文献出处：
引用格式：
[1]Chao Liu;Yun Wang;Yuyang Dao;Shuting Wang;Fei Hou;Zhixian Yang;Pengjie Liu;Juan Lv;Ling Lv;Gaofeng Li;Youjun Zhou;Zhiyong Deng-.Upregulation of CENPM facilitates lung adenocarcinoma progression via PI3K/AKT/mTOR signaling pathway)[J].生物化学与生物物理学报（英文版）,2022(01):99-112
A类：
Centromere,taset,abrogates
B类：
Upregulation,CENPM,facilitates,lung,adenocarcinoma,progression,via,PI3K,AKT,mTOR,signaling,pathway,protein,essential,chromosome,separation,during,mitosis,However,roles,LUAD,metastasis,remain,unknown,this,study,aimed,explore,effects,well,underlying,mechanisms,We,analyzed,expression,correlation,clinicopathological,characteristics,using,GEO,chip,datasets,TCGA,further,investigated,impact,vitro,vivo,silico,analysis,qRT,revealed,that,upregulated,compared,normal,tissues,Via,gain,loss,function,assays,found,promotes,cell,cycle,proliferation,migration,invasion,inhibits,apoptosis,showed,growth,A549,xenografts,Furthermore,can,phosphorylation,rather,than,directly,affect,content,Inhibition,activity,promoting,Taken,together,these,results,plays,important,may,be,promising,therapeutic,target
AB值：
0.490626
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