The spatiotemporal relationships in high-resolution during odontogenesis remain poorly understood.We report a cell lineage and atlas of developing mouse teeth.We performed a large-scale(92,688 cells)single cell RNA sequencing,tracing the cell trajectories during odontogenesis from embryonic days 10.5 to 16.5.Combined with an assay for transposase-accessible chromatin with high-throughput sequencing,our results suggest that mesenchymal cells show the specific transcriptome profiles to distinguish the tooth types.Subsequently,we identified key gene regulatory networks in teeth and bone formation and uncov-ered spatiotemporal patterns of odontogenic mesenchymal cells.CD24+and Plac8+cells from the mes-enchyme at the bell stage were distributed in the upper half and preodontoblast layer of the dental papilla,respectively,which could individually induce nonodontogenic epithelia to form tooth-like struc-tures.Specifically,the Plac8+tissue we discovered is the smallest piece with the most homogenous cells that could induce tooth regeneration to date.Our work reveals previously unknown heterogeneity and spatiotemporal patterns of tooth germs that may lead to tooth regeneration for regenerative dentistry.

Yaofeng Wang;Yifan Zhao;Shubin Chen;Xiaoming Chen;Yanmei Zhang;Hong Chen;Yuansong Liao;Jiashu Zhang;Di Wu;Hongxing Chu;Hongying Huang;Caixia Wu;Shijuan Huang;Huichao Xu;Bei Jia;Jie Liu;Bo Feng;Zhonghan Li;Dajiang Qin;Duanqing Pei;Jinglei Cai

Innovation Centre for Advanced Interdisciplinary Medicine,The Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou 510799,China;CAS Key Laboratory of Regenerative Biology,GIBH-CUHK Joint Research Laboratory on Stem Cell and Regenerative Medicine,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510530,China;Centre for Regenerative Medicine and Health,Hong Kong Institute of Science&Innovation,Chinese Academy of Sciences,Hong Kong,China;Bioland Laboratory,Guangzhou Regenerative Medicine and Health Guangdong Laboratory,Guangzhou 510005,China;Guangdong Provincial People's Hospital Ganzhou Hospital,Ganzhou Municipal Hospital,Ganzhou 341099,China;State Key Laboratory of Oral Disease,West China Hospital of Stomatology,Center of Growth Metabolism and Aging,Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610041,China;Department of Regeneration Medicine,School of Pharmaceutical Science,Jilin University,Changchun 130012,China;Department of Periodontics and Implantology,Stomatological Hospital,Southern Medical University(Guangdong Provincial Stomatological Hospital),Guangzhou 510515,China;Animal Center,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510530,China;The Center for Prenatal and Hereditary Disease Diagnosis,Department of Obstetrics and Gynecology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of Obstetrics and Gynecology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;School of Biomedical Sciences,The Chinese University of Hong Kong,Hong Kong,China;Laboratory of Cell Fate Control,School of Life Sciences,Westlake University,Hangzhou 310024,China

科学通报（英文版）

[1]Yaofeng Wang;Yifan Zhao;Shubin Chen;Xiaoming Chen;Yanmei Zhang;Hong Chen;Yuansong Liao;Jiashu Zhang;Di Wu;Hongxing Chu;Hongying Huang;Caixia Wu;Shijuan Huang;Huichao Xu;Bei Jia;Jie Liu;Bo Feng;Zhonghan Li;Dajiang Qin;Duanqing Pei;Jinglei Cai-.Single cell atlas of developing mouse dental germs reveals populations of CD24+and Plac8+odontogenic cells)[J].科学通报（英文版）,2022(11):1154-1169

germs,CD24+and,Plac8+odontogenic,uncov,Plac8+cells,enchyme,preodontoblast,nonodontogenic,Plac8+tissue

Single,atlas,developing,mouse,dental,reveals,populations,spatiotemporal,relationships,high,resolution,during,odontogenesis,remain,poorly,understood,We,report,lineage,teeth,performed,large,scale,single,sequencing,tracing,trajectories,from,embryonic,days,Combined,assay,transposase,accessible,chromatin,throughput,our,results,suggest,that,mesenchymal,show,specific,transcriptome,profiles,distinguish,tooth,types,Subsequently,identified,key,regulatory,networks,bone,formation,patterns,bell,stage,were,distributed,upper,half,layer,papilla,respectively,which,could,individually,induce,epithelia,like,struc,tures,Specifically,discovered,smallest,piece,most,homogenous,regeneration,date,Our,previously,unknown,heterogeneity,may,lead,regenerative,dentistry

0.528852