Juvenile hormone (JH) and 20-hydroxyecdysone (20E) coordinately regulate development and metamor-phosis in insects.Two JH intracellular receptors,methoprene-tolerant (Met) and germ-cell expressed(Gce),have been identified in the fruit fly Drosophila melanogaster.To investigate JH membrane signaling pathway without the interference from JH intracellular signaling,we characterized phosphoproteome profiles of the Met gce double mutant in the absence or presence of JH in both chronic and acute phases.Functioning through a potential receptor tyrosine kinase and phospholipase C pathway,JH membrane signaling activated protein kinase C (PKC) which phosphorylated ultraspiracle (USP) at Set35,the PKC phosphorylation site required for the maximal action of 20E through its nuclear receptor complex EcR-USP.The uspS35A mutant,in which Ser was replaced with Ala at position 35 by genome editing,showed decreased expression of Halloween genes that are responsible for ecdysone biosynthesis and thus atten-uated 20E signaling that delayed developmental timing.The uspS35A mutant also showed lower Yorkie activity that reduced body size.Altogether,JH membrane signaling phosphorylates USP at Ser35 and thus potentiates 20E action that regulates the normal fly development.This study helps better understand the complex JH signaling network.

Yue Gao;Suning Liu;Qiangqiang Jia;Lixian Wu;Dongwei Yuan;Emma Y.Li;Qili Feng;Guirong Wang;Subba R.Palli;Jian Wang;Sheng Li

Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technology & School of Life Sciences,South China Normal University,Guangzhou 510631,China;Guangmeiyuan R&D Center,Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,South China Normal University,Meizhou 514779,China;International Department,The Affiliated High School of South China Normal University,Guangzhou 510631,China;Lingnan Guangdong Laboratory of Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China;Department of Entomology,College of Agriculture,Food and Environment,University of Kentucky,Lexington 40546,USA;Department of Entomology,University of Maryland,College Park 20742,USA

科学通报（英文版）

[1]Yue Gao;Suning Liu;Qiangqiang Jia;Lixian Wu;Dongwei Yuan;Emma Y.Li;Qili Feng;Guirong Wang;Subba R.Palli;Jian Wang;Sheng Li-.Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila)[J].科学通报（英文版）,2022(02):186-197

phosis,Gce,gce,ultraspiracle,Set35,uspS35A,Halloween,Yorkie,Ser35

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