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典型文献
Calcium-dependent protein kinase CPK32 mediates calcium signaling in regulating Arabidopsis flowering time
文献摘要:
Appropriate flowering time is critical for the reproductive success of plant species.Emerging evidence indicates that calcium may play an important role in the regulation of flowering time.However,the underlying molecular mechanisms remain unclear.In this study,we demonstrate that calcium-dependent protein kinase 32 (CPK32) regulates flowering time by affecting the alternative polyadenylation of FLOWERING CONTROL LOCUS A (FCA) and altering the transcription of FLOWERING LOCUS C(FLC),a central repressor of flowering time.The knockdown of CPK32 results in an obvious late flowering phenotype and dramatically enhanced FLC transcription.CPK32 interacts with FCA,and phosphorylates the serine592 of FCA in a Ca2+-dependent manner.Moreover,the ratio of abundance of the FCA transcripts(FCA-D and FCA-P) changes significantly in the cpk32 mutant,which subsequently affects FLC expression and consequently regulates floral transition.The present evidence demonstrates that CPK32 modulates flowering time by regulating FCA alternative polyadenylation and consequent FLC expression.
文献关键词:
作者姓名:
Xidong Li;Limei Chen;Li Yao;Junjie Zou;Jie Hao;Weihua Wu
作者机构:
State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,China;Center for Crop Functional Genomics and Molecular Breeding,China Agricultural University,Beijing 100193,China;Syngenta Biotechnology China Co.,Ltd.,Beijing 102206,China;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China
引用格式:
[1]Xidong Li;Limei Chen;Li Yao;Junjie Zou;Jie Hao;Weihua Wu-.Calcium-dependent protein kinase CPK32 mediates calcium signaling in regulating Arabidopsis flowering time)[J].国家科学评论(英文版),2022(01):51-60
A类:
CPK32,serine592,cpk32
B类:
Calcium,dependent,protein,kinase,mediates,calcium,signaling,regulating,Arabidopsis,flowering,Appropriate,critical,reproductive,success,plant,species,Emerging,evidence,indicates,that,may,play,important,role,regulation,However,underlying,molecular,mechanisms,remain,unclear,In,this,study,regulates,by,affecting,alternative,polyadenylation,FLOWERING,CONTROL,LOCUS,FCA,altering,transcription,FLC,central,repressor,knockdown,results,obvious,phenotype,dramatically,enhanced,interacts,phosphorylates,Ca2+,manner,Moreover,ratio,abundance,transcripts,changes,significantly,mutant,which,subsequently,affects,expression,consequently,floral,transition,present,demonstrates,modulates
AB值:
0.505397
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