Halobenzoquinones(HBQs)are emerging disinfection byproducts(DBPs)with a widespread presence in drinking water that exhibit much higher cytotoxicity than regulated DBPs.However,the developmental neurotoxicity of HBQs has not been studied in vivo.In this work,we studied the neurotoxicity of HBQs on zebrafish embryos,after exposure to vary-ing concentrations(0-8 μmol/L)of three HBQs,2,5-dichloro-1,4-benzoquinone(2,5-DCBQ),2,6-dichloro-1,4-benzoquinone(2,6-DCBQ),and 2,5-dibromo-1,4-benzoquinone(2,5-DBBQ)for 4 to 120 hr post fertilization(hpf).HBQ exposure significantly decreased the locomo-tor activity of larvae,accompanied by significant reduction of neurotransmitters(dopamine and γ-aminobutyric acid)and acetylcholinesterase activity.Furthermore,the expression of genes involved in neuronal morphogenesis(gfap,α1-tubulin,mbp,and syn-2α)were downreg-ulated by 4.4-,5.2-,3.0-,and 4.5-fold in the 5 μmol/L 2,5-DCBQ group and 2.0-,1.6-,2.1-,and 2.3-fold in the 5 μmol/L 2,5-DBBQ group,respectively.Transcriptomic analysis revealed that HBQ exposure affected the signaling pathways of neural development.This study demon-strates the significant neurotoxicity of HBQs in embryonic zebrafish and provides molecular evidence for understanding the potential mechanisms of HBQ neurotoxicity.

Xue Yang;Chang Wang;Lihua Yang;Qi Zheng;Qiongyu Liu;Nicholas J.P.Wawryk;Xing-Fana Li

Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances,School of Environment and Health,Jianghan University,Wuhan 430056,China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center,Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering,School of Marine Sciences,Sun Yat-Sen University,Zhuhai 519082,China;Division of Analytical and Environmental Toxicology,Department of Laboratory Medicine and Pathology,Faculty of Medicine and Dentistry,University of Alberta,Edmonton,Alberta,T6G 2G3,Canada

环境科学学报（英文版）

[1]Xue Yang;Chang Wang;Lihua Yang;Qi Zheng;Qiongyu Liu;Nicholas J.P.Wawryk;Xing-Fana Li-.Neurotoxicity and transcriptome changes in embryonic zebrafish induced by halobenzoauinone exposure)[J].环境科学学报（英文版）,2022(07):129-140

halobenzoauinone,Halobenzoquinones,DBBQ,HBQ,locomo,gfap,downreg

Neurotoxicity,transcriptome,changes,embryonic,zebrafish,induced,exposure,HBQs,are,emerging,disinfection,byproducts,DBPs,widespread,presence,drinking,water,that,exhibit,much,higher,cytotoxicity,than,regulated,However,developmental,neurotoxicity,has,not,been,studied,vivo,In,this,work,embryos,after,vary,concentrations,three,dichloro,DCBQ,dibromo,post,fertilization,hpf,significantly,decreased,tor,activity,larvae,accompanied,reduction,neurotransmitters,dopamine,aminobutyric,acid,acetylcholinesterase,Furthermore,expression,involved,neuronal,morphogenesis,tubulin,mbp,syn,were,fold,group,respectively,Transcriptomic,analysis,revealed,affected,signaling,pathways,neural,This,study,demon,strates,provides,molecular,evidence,understanding,potential,mechanisms

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