典型文献
Identification of a sensor histidine kinase(BfcK)controlling biofilm formation in Clostridium acetobutylicum
文献摘要：
Clostridium acetobutylicum has been extensively exploited to produce biofuels and solvents and its biofilm could dramatically improve the productivities.However,genetic control of C.acetobutylicum biofilm has not been dissected so far.Here,to identify potential genes controlling C acetobutylicum biofilm formation,over 40 gene candidates associated with extracellular matrix,cell surface,cell signaling or gene transcrip-tion,were tried to be disrupted to examine their individual impact.A total of 25 disruptants were finally obtained over years of attempts,for which biofilm and relevant phenotypes were characterized.Most of these disruptants formed robust biofilm still,or suffered both growth and biofilm defect.Only a strain with a disrupted histidine kinase gene(CA_C2730,designated bfcK in this study)abolished biofilm forma-tion without impairing cell growth or solvent production.Further analysis revealed that bfcK could con-trol flagellar biogenesis and cell motility at protein levels.The bfcK also appeared to repress the phosphorylation of a serine/threonine protein kinase(encoded by CA_C0404)that might negatively reg-ulate biofilm formation.Based on these findings,possible bfcK-mediated mechanisms for biofilm forma-tion were proposed.This is a big step toward understanding the biofilm formation in C.acetobutylicum and will help further engineering of its biofilm-based industrial processes.
文献关键词：
中图分类号：
作者姓名：
Dong Liu;Shikai Ge;Zhenyu Wang;Mengting Li;Wei Zhuang;Pengpeng Yang;Yong Chen;Hanjie Ying
作者机构：
State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816,China;School of Chemical Engineering and Energy,Zhengzhou University,Zhengzhou 450001,China
文献出处：
引用格式：
[1]Dong Liu;Shikai Ge;Zhenyu Wang;Mengting Li;Wei Zhuang;Pengpeng Yang;Yong Chen;Hanjie Ying-.Identification of a sensor histidine kinase(BfcK)controlling biofilm formation in Clostridium acetobutylicum)[J].中国化学工程学报（英文版）,2022(06):84-93
A类：
BfcK,acetobutylicum,disruptants,C2730,bfcK,C0404
B类：
Identification,sensor,histidine,kinase,controlling,biofilm,formation,Clostridium,has,been,extensively,exploited,produce,biofuels,solvents,its,could,dramatically,improve,productivities,However,genetic,dissected,far,Here,identify,potential,over,candidates,associated,extracellular,matrix,surface,signaling,transcrip,were,tried,disrupted,examine,their,individual,impact,total,finally,obtained,years,attempts,which,relevant,phenotypes,characterized,Most,these,formed,robust,still,suffered,both,growth,defect,Only,strain,CA,designated,this,study,abolished,without,impairing,production,Further,analysis,revealed,that,flagellar,biogenesis,motility,protein,levels,also,appeared,repress,phosphorylation,serine,threonine,encoded,by,might,negatively,reg,ulate,Based,findings,possible,mediated,mechanisms,proposed,This,big,step,toward,understanding,will,help,further,engineering,industrial,processes
AB值：
0.529914
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