典型文献
Continuous subcellular resolution three-dimensional imaging on intact macaque brain
文献摘要：
To decipher the organizational logic of complex brain circuits,it is important to chart long-distance path-ways while preserving micron-level accuracy of local network.However,mapping the neuronal projec-tions with individual-axon resolution in the large and complex primate brain is still challenging.Herein,we describe a highly efficient pipeline for three-dimensional mapping of the entire macaque brain with subcellular resolution.The pipeline includes a novel poly-N-acryloyl glycinamide (PNAGA)-based embedding method for long-term structure and fluorescence preservation,high-resolution and rapid whole-brain optical imaging,and image post-processing.The cytoarchitectonic information of the entire macaque brain was acquired with a voxel size of 0.32 μm × 0.32 μm × 10 μm,showing its anatomical structure with cell distribution,density,and shape.Furthermore,thanks to viral labeling,individual long-distance projection axons from the frontal cortex were for the first time reconstructed across the entire brain hemisphere with a voxel size of 0.65 μm × 0.65 μm × 3 μm.Our results show that individual cortical axons originating from the prefrontal cortex simultaneously target multiple brain regions,including the visual cortex,striatum,thalamus,and midbrain.This pipeline provides an efficient method for cellular and circuitry investigation of the whole macaque brain with individual-axon resolu-tion,and can shed light on brain function and disorders.
文献关键词：
中图分类号：
作者姓名：
Can Zhou;Xiaoquan Yang;Shihao Wu;Qiuyuan Zhong;Ting Luo;Anan Li;Guangcai Liu;Qingtao Sun;Pan Luo;Lei Deng;Hong Ni;Chaozhen Tan;Jing Yuan;Qingming Luo;Xintian Hu;Xiangning Li;Hui Gong
作者机构：
Britton Chance Center for Biomedical Photonics,Wuhan National Laboratory for Optoelectronics,MoE Key Laboratory for Biomedical Photonics,Huazhong University of Science and Technology,Wuhan 430074,China;HUST-Suzhou Institute for Brainsmatics,JITRI,Suzhou 215123,China;Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China;Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province,Kunming Institute of Zoology,Kunming 650223,China;CAS Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,China;UST-Suzhou Institute for Brainsmatics, JITRI, Suzhou 215123, China;Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan 430074, China;HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou 215123, China;CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
文献出处：
引用格式：
[1]Can Zhou;Xiaoquan Yang;Shihao Wu;Qiuyuan Zhong;Ting Luo;Anan Li;Guangcai Liu;Qingtao Sun;Pan Luo;Lei Deng;Hong Ni;Chaozhen Tan;Jing Yuan;Qingming Luo;Xintian Hu;Xiangning Li;Hui Gong-.Continuous subcellular resolution three-dimensional imaging on intact macaque brain)[J].科学通报（英文版）,2022(01):85-96
A类：
acryloyl,glycinamide,PNAGA,cytoarchitectonic
B类：
Continuous,subcellular,resolution,three,dimensional,imaging,intact,macaque,To,decipher,organizational,logic,complex,circuits,important,chart,long,distance,path,ways,while,preserving,micron,level,accuracy,local,network,However,mapping,neuronal,tions,individual,large,primate,still,challenging,Herein,describe,highly,efficient,pipeline,entire,includes,novel,poly,embedding,method,term,structure,fluorescence,preservation,rapid,whole,optical,image,post,processing,information,was,acquired,voxel,size,showing,anatomical,distribution,density,shape,Furthermore,thanks,viral,labeling,projection,axons,from,cortex,were,first,reconstructed,across,hemisphere,Our,results,that,cortical,originating,prefrontal,simultaneously,target,multiple,regions,including,visual,striatum,thalamus,midbrain,This,provides,circuitry,investigation,can,shed,light,function,disorders
AB值：
0.547179
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