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典型文献
Rapid full-color Fourier ptychographic microscopy via spatially filtered color transfer
文献摘要:
Full-color imaging is of critical importance in digital pathology for analyzing labeled tissue sections.In our pre-vious cover story[Sci.China:Phys.,Mech.Astron.64,114211(2021)],a color transfer approach was imple-mented on Fourier ptychographic microscopy(FPM)for achieving high-throughput full-color whole slide imaging without mechanical scanning.The approach was able to reduce both acquisition and reconstruction time of FPM by three-fold with negligible trade-off on color accuracy.However,the method cannot properly stain samples with two or more dyes due to the lack of spatial constraints in the color transfer process.It also requires a high computation cost in histogram matching of individual patches.Here we report a modified full-color imaging algorithm for FPM,termed color-transfer filtering FPM(CFFPM).In CFFPM,we replace the original histogram matching process with a combination of block processing and trilateral spatial filtering.The former step reduces the search of the solution space for colorization,and the latter introduces spatial con-straints that match the low-resolution measurement.We further adopt an iterative process to refine the results.We show that this method can perform accurate and fast color transfer for various specimens,including those with multiple stains.The statistical results of 26 samples show that the average root mean square error is only 1.26%higher than that of the red-green-blue sequential acquisition method.For some cases,CFFPM outperforms the sequential method because of the coherent artifacts introduced by dust particles.The reported CFFPM strategy provides a turnkey solution for digital pathology via computational optical imaging.
文献关键词:
作者姓名:
JIURUN CHEN;AIYE WANG;AN PAN;GUOAN ZHENG;CAIWEN MA;BAOLI YAO
作者机构:
Xi'an Institute of Optics and Precision Mechanics,Chinese Academy of Sciences,Xi'an 710119,China;University of Chinese Academy of Sciences,Beijing 100049,China;CAS Key Laboratory of Space Precision Measurement Technology,Xi'an 710119,China;Department of Biomedical Engineering,University of Connecticut,Storrs,Connecticut 06269,USA
引用格式:
[1]JIURUN CHEN;AIYE WANG;AN PAN;GUOAN ZHENG;CAIWEN MA;BAOLI YAO-.Rapid full-color Fourier ptychographic microscopy via spatially filtered color transfer)[J].光子学研究(英文),2022(10):2410-2421
A类:
ptychographic,Astron,CFFPM,trilateral,colorization
B类:
Rapid,full,Fourier,microscopy,via,spatially,filtered,transfer,Full,imaging,critical,importance,digital,pathology,analyzing,labeled,tissue,sections,In,pre,vious,cover,story,Sci,China,Phys,Mech,approach,was,imple,mented,achieving,throughput,whole,slide,without,mechanical,scanning,able,both,acquisition,reconstruction,by,three,fold,negligible,trade,off,accuracy,However,method,cannot,properly,samples,two,more,dyes,due,lack,constraints,It,also,requires,cost,histogram,matching,individual,patches,Here,modified,algorithm,termed,filtering,replace,original,combination,block,processing,former,step,reduces,search,space,latter,introduces,that,low,resolution,measurement,We,further,adopt,iterative,refine,results,show,this,accurate,fast,various,specimens,including,those,multiple,stains,statistical,average,root,mean,square,error,only,higher,than,green,blue,sequential,For,some,cases,outperforms,because,coherent,artifacts,introduced,dust,particles,reported,strategy,provides,turnkey,computational,optical
AB值:
0.544651
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