典型文献
Axial gradient excitation accelerates volumetric imaging of two-photon microscopy
文献摘要：
Two-photon excitation fluorescence microscopy(TPM),owing to its capacity for subcellular resolution,intrinsic optical sectioning,and superior penetration depth in turbid samples,has revolutionized biomedical research.However,its layer-by-layer scanning to form a three-dimensional image inherently limits the volumetric imaging speed and increases phototoxicity significantly.In this study,we develop a gradient excitation technique to accel-erate TPM volumetric imaging.The axial positions of the flluorophores can be decoded from the intensity ratio of the paired images obtained by sequentially exciting the specimen with two axially elongated two-photon beams of complementary gradient intensities.We achieved a 0.63 μm axial localization precision and demonstrate the flexibility of the gradient TPM on various sparsely labeled samples,including bead phantoms,mouse brain tis-sues,and live macrophages.Compared with traditional TPM,our technique improves volumetric imaging speed(by at least sixfold),decreases photobleaching(i.e.,less than 2.07±2.89％in 25 min),and minimizes photodamages.
文献关键词：
中图分类号：
作者姓名：
YUFENG GAO;XIANYUAN XIA;LINA LIU;TING WU;TINGAI CHEN;JIA YU;ZHILI XU;LIANG WANG;FEI YAN;ZHUO DU;JUN CHU;YANG ZHAN;BO PENG;HUI LI;WEI ZHENG
作者机构：
Research Center for Biomedical Optics and Molecular Imaging,Shenzhen Key Laboratory for Molecular Imaging,Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;CAS Key Laboratory of Health Informatics,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;Paul C.Lauterbur Research Center for Biomedical Imaging,Institute of Biomedical and Health Engineering,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;State Key Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China;Key Laboratory of Genetic Network Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China;Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;Centre for Micro Nano Systems and Bionic Medicine,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China
文献出处：
引用格式：
[1]YUFENG GAO;XIANYUAN XIA;LINA LIU;TING WU;TINGAI CHEN;JIA YU;ZHILI XU;LIANG WANG;FEI YAN;ZHUO DU;JUN CHU;YANG ZHAN;BO PENG;HUI LI;WEI ZHENG-.Axial gradient excitation accelerates volumetric imaging of two-photon microscopy)[J].光子学研究（英文）,2022(03):687-696
A类：
flluorophores,photobleaching,photodamages
B类：
Axial,gradient,excitation,accelerates,volumetric,imaging,two,photon,microscopy,Two,fluorescence,TPM,owing,capacity,subcellular,resolution,intrinsic,optical,sectioning,superior,penetration,depth,turbid,samples,has,revolutionized,biomedical,research,However,layer,by,scanning,form,three,dimensional,inherently,limits,speed,increases,phototoxicity,significantly,In,this,study,develop,technique,positions,decoded,from,intensity,paired,images,obtained,sequentially,exciting,specimen,axially,elongated,beams,complementary,intensities,We,achieved,localization,precision,demonstrate,flexibility,various,sparsely,labeled,including,bead,phantoms,mouse,brain,tis,sues,live,macrophages,Compared,traditional,our,improves,least,sixfold,decreases,less,than,minimizes
AB值：
0.63806
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