Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM)),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)were analyzed.The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored.Methods:National standard method and HPLC were used for active ingredient analysis.MTT,cell flow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apop-tosis,and its underlying molecular mechanism.Results:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester cat-echins,non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM.The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM.Three T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells,especially YT-CM and GT-CM.Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax,Bcl-2 and Caspase-3/9.YT-CM and GT-CM had better ability to change the signal pathway than BT-CM.Conclusion:In short,T-CMs,which combined different degrees of fermentation tea with C.maxima,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and GT-CM,are healthy drinks that help to prevent and treat liver cancer.

Shuai Wen;Ran An;Dongli Li;Junxi Cao;Zhigang Li;Wenji Zhang;Ruohong Chen;Qiuhua Li;Xingfei Lai;Lingli Sun;Shili Sun

Tea Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation&Utilization,Guangzhou,510640,China;School of Biotechnology and Health Sciences,Wuyi University,Jiangmen 529020,China;International Healthcare Innovation Institute(Jiangmen),Jiangmen 529040,China

中草药（英文版）

[1]Shuai Wen;Ran An;Dongli Li;Junxi Cao;Zhigang Li;Wenji Zhang;Ruohong Chen;Qiuhua Li;Xingfei Lai;Lingli Sun;Shili Sun-.Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro)[J].中草药（英文版）,2022(03):449-458

apop,echins,Bel7402

Tea,Citrus,maxima,complex,induces,apoptosis,human,liver,cancer,cells,via,PI3K,AKT,mTOR,pathway,vitro,Objective,In,this,study,black,tea,BT,yellow,YT,green,GT,subjects,active,ingredient,content,antioxidant,activity,three,were,analyzed,effects,CMs,its,mechanism,further,explored,Methods,National,standard,method,HPLC,used,analysis,MTT,flow,cytometry,blot,proliferation,underlying,molecular,Results,polyphenols,free,amino,acids,catechins,caffeine,was,significantly,higher,than,that,capacity,also,stronger,Three,had,inhibiting,arresting,cycle,inducing,HepG2,especially,showed,activated,signaling,regulated,expression,levels,related,proteins,Bax,Bcl,Caspase,better,ability,change,Conclusion,short,which,combined,different,degrees,fermentation,rich,nutrients,biologically,substances,are,healthy,drinks,help,prevent,treat

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