典型文献
Qilan preparation(芪蓝颗粒)inhibits proliferation and induces apoptosis by down-regulating microRNA-21 in human Tca8113 tongue squamous cell carcinoma cells
文献摘要：
OBJECTIVE:The aim of this study was to examine the antitumor effects of Qilan preparation(芪蓝颗粒)on oral squamous cell carcinoma(OSCC)in vitro and to investigate its underlying mechanisms of action.METHODS:Cell proliferation,cell cycle distribution and apoptosis were examined using cell counting kit-8(CCK8)and flow cytometry(FCM).The expression of PTEN and PDCD4 were determined by western blot.Changes in miR-21 levels were quantified using TaqMan stem-loop real-time PCR.After miR-21 was transiently transfected into Tca8113 cells using Lipofectamine?3000,cell proliferation,apoptosis and miR-21 and PDCD4 expression levels were measured.RESULTS:Qilan preparation inhibited Tca8113 cell growth in a dose-and time-dependent manner by inducing apoptosis and cell cycle arrest in S-phase,decreasing miR-21 levels and increasing PTEN and PDCD4 expression.MiR-21 overexpression reversed the Qilan preparation-induced suppression of cell proliferation and induction of apoptosis while also blocking the increase in PDCD4.CONCLUSIONS:Our study revealed,for the first time,the ability of Qilan preparation to suppress TSCC cell growth and elucidated that Qilan preparation elicits its anti-cancer actions via either the miR-21/PDCD4 or PTEN pathway.
文献关键词：
中图分类号：
作者姓名：
DING Jiamin;XING Yifeng;CHEN Zuoliang;CHEN Wanlu;MA Zhongxiong;XIE Yunde;ZHOU Lin
作者机构：
Department of Oral Mucosal Diseases,Affiliated Stomatological Hospital of Fujian Medical University,Fuzhou 350000,China;School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350000,China;Department of Oral Mucosal Diseases,Xiamen Stomatological Hospital,School of Stomatology,Fujian Medical University,Xiamen 361003,China;Department of Implantology,Affiliated Stomatological Hospital of Fujian Medical University,Fuzhou 350001,China
文献出处：
引用格式：
[1]DING Jiamin;XING Yifeng;CHEN Zuoliang;CHEN Wanlu;MA Zhongxiong;XIE Yunde;ZHOU Lin-.Qilan preparation(芪蓝颗粒)inhibits proliferation and induces apoptosis by down-regulating microRNA-21 in human Tca8113 tongue squamous cell carcinoma cells)[J].中医杂志（英文版）,2022(05):693-700
A类：
Qilan
B类：
preparation,inhibits,proliferation,induces,apoptosis,by,down,regulating,microRNA,human,Tca8113,tongue,squamous,carcinoma,cells,OBJECTIVE,aim,this,study,was,antitumor,effects,oral,OSCC,vitro,investigate,underlying,mechanisms,METHODS,Cell,cycle,distribution,were,examined,using,counting,kit,CCK8,flow,cytometry,FCM,PTEN,PDCD4,determined,western,blot,Changes,miR,levels,quantified,TaqMan,stem,loop,real,After,transiently,transfected,into,Lipofectamine,measured,RESULTS,inhibited,growth,dose,dependent,manner,inducing,arrest,phase,decreasing,increasing,MiR,overexpression,reversed,induced,suppression,induction,while,also,blocking,increase,CONCLUSIONS,Our,revealed,first,ability,TSCC,elucidated,that,elicits,cancer,actions,via,either,pathway
AB值：
0.533751
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