Objective:Qili Qiangxin(QLQX),a compound herbal medicine formula,is used effectively to treat conges-tive heart failure in China.However,the molecular mechanisms of the cardioprotective effect are still unclear.This study explores the cardioprotective effect and mechanism of QLQX using the hypoxia-reoxygenation(H/R)-induced myocardial injury model.Methods:The main chemical constituents of QLQX were analyzed using high-performance liquid chromatography-evaporative light-scattering detection.The model of H/R-induced myocardial injury in H9c2 cells was developed to simulate myocardial ischemia-reperfusion injury.Apoptosis,autophagy,and generation of reactive oxygen species(ROS)were measured to assess the protective effect of QLQX.Proteins related to autophagy,apoptosis and signalling pathways were detected using Western blotting.Results:Apoptosis,autophagy and the excessive production of ROS induced by H/R were significantly reduced after treating the H9c2 cells with QLQX.QLQX treatment at concentrations of 50 and 250 μg/mL caused significant reduction in the levels of LC3Ⅱ and p62 degradation(P＜0.05),and also suppressed the AMPK/mTOR signalling pathway.Furthermore,the AMPK inhibitor Compound C(at 0.5 μmol/L),and QLQX(250 μg/mL)significantly inhibited H/R-induced autophagy and apoptosis(P＜0.01),while AICAR(an AMPK activator,at 0.5 mmol/L)increased cardiomyocyte apoptosis and autophagy and abol-ished the anti-apoptotic effect of QLQX.Similar phenomena were also observed on the expressions of apoptotic and autophagic proteins,demonstrating that QLQX reduced the apoptosis and autophagy in the H/R-induced injury model via inhibiting the AMPK/mTOR pathway.Moreover,ROS scavenger,N-Acetyl-L-cysteine(NAC,at 2.5 mmol/L),significantly reduced H/R-triggered cell apoptosis and autop-hagy(P＜0.01).Meanwhile,NAC treatment down-regulated the ratio of phosphorylation of AMPK/AMPK(P＜0.01),which showed a similar effect to QLQX.Conclusion:QLQX plays a cardioprotective role by alleviating apoptotic and autophagic cell death through inhibition of the ROS/AMPK/mTOR signalling pathway.

Cai-lian Fan;Wan-jun Cai;Meng-nan Ye;Miao Chen;Yi Dai

Department of Cardiology,the First Affiliated Hospital of Jinan University,Jinan University,Guangzhou 510632,Guangdong Province,China;College of Pharmacy,Jinan University,Guangzhou 510632,Guangdong Province,China

结合医学学报（英文版）

[1]Cai-lian Fan;Wan-jun Cai;Meng-nan Ye;Miao Chen;Yi Dai-.Qili Qiangxin,a compound herbal medicine formula,alleviates hypoxia-reoxygenation-induced apoptotic and autophagic cell death via suppression of ROS/AMPK/mTOR pathway in vitro)[J].结合医学学报（英文版）,2022(04):365-375

Qili,Qiangxin,QLQX,conges,abol,autop,hagy

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