Objective:We aimed to observe the effects of loganin(Log)on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK)signaling in obese mice.Methods:A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR)mice.Body weight was measured weekly and fasting blood glucose(FBG)was determined every 2 weeks.Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed.The serum levels of total cholesterol(TC),triglyceride,high-density lipoprotein-cholesterol,low-density lipoprotein-cholesterol(LDL-C),and free fatty acids(FFA)were measured.The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting,and gut microbiota were characterized using 16S rDNA sequencing.Results:Log significantly decreased the body weight and the FBG in obese mice(P＜.05),and it could restore FBG to normal levels.The total cholesterol,LDL-C,and FFA levels were significantly reduced by Log compared with the obese controls(TC:P=.0020;LDL-C:P=.0233;FFA:P=.0127),and the glucose tolerance of animals was significantly improved(P=.0477).The western blot results showed that Log could upregulate the protein expression of Adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPKα),Sirtuin 1(SIRT1),and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1α)in skeletal muscle tissue of obese mice.16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.Conclusions:Log was effective in reducing body weight and improving glucolipid metabolism in obese mice,probably through activating AMPK signaling and regulating intestinal microbial diversity.

Bingrui Xu;Zimengwei Ye;Tian Tian;Ruyuan Zhu;Chenyue Liu;Xin Fang;Dongwei Zhang;Min Fu;Sihua Gao;Dandan Zhao

School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,102488,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,100700,China;Department of Endocrinology,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing,100029,China;Research Institute of McGill University Health Center,McGill University,Montreal,H4A 3J1,Canada

中医科学杂志（英文）

[1]Bingrui Xu;Zimengwei Ye;Tian Tian;Ruyuan Zhu;Chenyue Liu;Xin Fang;Dongwei Zhang;Min Fu;Sihua Gao;Dandan Zhao-.Loganin regulates glycolipid metabolism by influencing intestinal microbiota and AMPK signaling in obese mice)[J].中医科学杂志（英文）,2022(03):321-329

Loganin,loganin,glucolipid

regulates,glycolipid,metabolism,by,influencing,intestinal,microbiota,AMPK,signaling,obese,mice,Objective,We,aimed,observe,effects,serum,levels,probe,mechanisms,focusing,activated,kinase,Methods,high,diet,was,given,consecutive,weeks,generate,obesity,model,institute,cancer,research,ICR,Body,weight,measured,weekly,fasting,blood,glucose,FBG,determined,every,Both,tolerance,intraperitoneal,insulin,were,performed,total,cholesterol,triglyceride,density,lipoprotein,low,LDL,free,fatty,acids,FFA,expression,key,proteins,pathway,skeletal,muscle,tissue,detected,immunoblotting,gut,characterized,16S,rDNA,sequencing,Results,significantly,decreased,body,could,restore,normal,reduced,compared,controls,animals,improved,western,results,showed,that,upregulate,Adenosine,monophosphate,Sirtuin,SIRT1,peroxisome,proliferator,receptor,gamma,coactivator,alpha,PGC1,indicated,diversity,feces,altered,floral,composition,Conclusions,effective,reducing,improving,probably,through,activating,regulating,microbial

0.496415