典型文献
Zebrafish Foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression
文献摘要：
Chamber maturation is a significant process in cardiac development.Disorders of this crucial process lead to a range of congenital heart defects.Foxc1a is a critical transcription factor reported to regulate the specification of cardiac progenitor cells.However,little is known about the role of Foxc1a in modulating chamber maturation.Previously,we reported that foxc1a-null zebrafish embryos exhibit disrupted heart structures and functions.In this study,we observe that ventricle structure and cardiomyocyte proliferation are abolished during chamber maturation in foxc1a-null zebrafish embryos.To observe the endogenous localization of Foxc1a in the hearts of living embryos,we insert eyfp at the foxc1a genomic locus using TALEN.Analysis of the knockin zebrafish show that foxc1a is widely expressed in ventricular car-diomyocytes during chamber development.Cardiac RNA sequencing analysis reveals the downregulated expression of the Hippo signaling effector wwtr1.Dual-luciferase and chromatin immunoprecipitation as-says reveal that Foxc1a can bind directly to three sites in the wwtr1 promoter region.Furthermore,wwtr1 mRNA overexpression is sufficient to reverse the ventricle defects during chamber maturation.Conditional overexpression of nkx2.5 also partially rescues the ventricular defects during chamber development.These findings demonstrate that wwtr1 and nkx2.5 are direct targets of Foxc1a during ventricular chamber maturation.
文献关键词：
中图分类号：
作者姓名：
Luqingqing He;Qinxin Zhang;Dongya Jiang;Yunfeng Zhang;Yuxuan Wei;Yuxi Yang;Nan Li;Shuang Wang;Yunyun Yue;Qingshun Zhao
作者机构：
Model Animal Research Center,Medical School,Nanjing University,Nanjing,Jiangsu 210061,China;Department of Prenatal Diagnosis,Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital,Nanjing,Jiangsu 210004,China;School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing,Jiangsu 210009,China
文献出处：
引用格式：
[1]Luqingqing He;Qinxin Zhang;Dongya Jiang;Yunfeng Zhang;Yuxuan Wei;Yuxi Yang;Nan Li;Shuang Wang;Yunyun Yue;Qingshun Zhao-.Zebrafish Foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression)[J].遗传学报,2022(06):559-568
A类：
Foxc1a,wwtr1,nkx2,foxc1a,eyfp,diomyocytes
B类：
Zebrafish,controls,ventricular,chamber,maturation,by,directly,regulating,Chamber,significant,process,cardiac,development,Disorders,this,crucial,lead,range,congenital,defects,critical,transcription,reported,specification,progenitor,cells,However,little,known,about,role,modulating,Previously,that,null,zebrafish,embryos,exhibit,disrupted,structures,functions,In,study,observe,ventricle,cardiomyocyte,proliferation,are,abolished,during,To,endogenous,localization,hearts,living,insert,genomic,locus,using,TALEN,Analysis,knockin,show,widely,expressed,Cardiac,sequencing,analysis,reveals,downregulated,Hippo,signaling,effector,Dual,luciferase,chromatin,immunoprecipitation,says,bind,three,sites,promoter,region,Furthermore,overexpression,sufficient,reverse,Conditional,also,partially,rescues,These,findings,demonstrate,targets
AB值：
0.466865
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
