The aquaporin 8(AQP8)is a small integral membrane protein that selectively transports water and other small uncharged solutes across cell plasma membranes.It has been demonstrated that AQP8 is ubiquitously present in various tissues and organs of mammals,and participates in many physiological and pathological processes.Recent studies showed that AQP8 is highly expressed in the columnar epithelial cells of mammalian colonic mucosa facing lumen,indicating that AQP8 plays potential roles in the physiology and pathophysiology of gastrointestinal tract.However,the role of AQP8 during gastrointestinal tract development is unclear.In the present study,RT-PCR results reveal that the zebrafish genome encodes three kinds of aqp8s(aqp8aa,aqp8ab,and aqp8b).We use whole mount in situ hybridization to describe aqp8 genes spatiotemporal expression pattern,and the results show that aqp8ab mRNA is detectable mainly in the zebrafish embryonic intestine.To reveal the details of aqp8ab distribution,histological sections are employed.Transverse sections indicate that aqp8ab mRNA expression is more intense in the layer lining the intestinal cavity.Knockout of aqp8ab using the CRISPR/Cas9 system induces intestine devel-opment defects and abnormal formation of intestinal lumen.In addition,aqp8ab mRNA significantly rescues the intestine defects in the aqp8ab mutant.These results indicate that aqp8ab is required in the intestine development of zebrafish.

Division of Gastrointestinal Surgery,Department of General Surgery,the First Affiliated Hospital,Nanjing Medical University,Nanjing 210029,China;The Affiliated Suqian First People's Hospital of Nanjing Medical University,Suqian 223800,China;School of Life Science,Co-innovation Center of Neuroregeneration,Key Laboratory of Neurogeneration of Jiangsu and MOE,Nantong 226001,China

[1]Shiping Wang;Yinyin Qin;Jiajing Sheng;Xuchu Duan;Lizong Shen;Dong Liu-.Aquaporin 8ab is required in zebrafish embryonic intestine development)[J].生物化学与生物物理学报（英文版）,2022(07):952-960

8ab,uncharged,aqp8s,aqp8aa,aqp8ab,aqp8b,aqp8

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