FUSCA3 (FUS3) is a member of B3-domain transcription factor family and master regulator of seed development. It has potential roles in hormone biosynthesis and signaling pathways and therefore plays diverse roles in plant life cycle, especially in seed germination, dormancy, embryo formation, seed and fruit development, and maturation. However, there is limited information about its functions in seed and fruit development of grapevine. In this study, we expressed VvFUS3 in tomato for its functional characterization. Overexpression of VvFUS3 in tomato led to a reduction in seed number and seed weight without affecting the fruit size. Histological analysis found that both cell expansion and cell division in transgenic seed and fruit pericarp have been affected. However, there were no obvious differences in pollen size, shape, and viability, suggesting that VvFUS3 affects seed development but not the pollen grains. Moreover, the expression of several genes with presumed roles in seed development and hormone signaling pathways was also influenced by VvFUS3. These results suggest that VvFUS3 is involved in hormonal signaling pathways that regulate seed number and size. In conclusion, our study provides novel preliminary information about the pivotal roles of VvFUS3 in seed and fruit development and these findings can potentially serve as a reference for molecular breeding of seedless grapes.

Bilal Ahmad;Songlin Zhang;Jin Yao;Shengyue Chai;Vivek Yadav;Habib-ur-Rehman Athar;Mati Ur Rahman;Li Wang;Xiping Wang

State Key Laboratory of Crop Stress Biology in Arid Areas,College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China;Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture,Northwest A&F University,Yangling,Shaanxi 712100,China;Institute of Pure and Applied Biology,Bahauddin Zakariya University,Multan 60800,Pakistan;College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710119,China;College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China

园艺学报（英文版）

[1]Bilal Ahmad;Songlin Zhang;Jin Yao;Shengyue Chai;Vivek Yadav;Habib-ur-Rehman Athar;Mati Ur Rahman;Li Wang;Xiping Wang-.Ectopic expression of VvFUS3, B3-domain transcription factor, in tomato influences seed development via affecting endoreduplication and hormones)[J].园艺学报（英文版）,2022(03):351-360

VvFUS3,endoreduplication,seedless

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