Disruption of morphogenesis,an essential process in organismal development,can lead to disruption of biological processes,reduction in fitness,or even death of an organism.The roles of lethal giant larvae(Lgl)protein in maintaining tissue organization have been studied extensively in mammals,but little is known about this gene's roles in promoting correct tissue morphogenesis in insects.In this study,we identified an Lgl or-tholog in Locusta migratoria.RT-qPCR results revealed that LmLgl was constitutively ex-pressed during third,fourth,and fifth instar nymphs.Furthermore,LmLgl showed highest expression in the ovary followed by wing pads,midgut,hindgut,Malpighian tubules,and foregut of the third-instar nymphs.To examine the role of LmLgl in L.migratoria devel-opment,RNA interference was performed during nymphal stages.Silencing of LmLgl in-creased body size but decreased body weight by 9.0％.Histological sections of the midgut revealed abnormal large masses of disordered epithelial cells in dsLmLgl-injected nymphs.In addition,downregulation of LmLgl transcript levels significantly altered the morpho-logical structure in midgut,resulting in the formation of tumor-like structures.Our results indicated that LmLgl may act as a tumor-suppressor gene,which plays an essential role in maintaining a normal morphological structure in the midgut of L.migratoria.Our results also suggest that LmLgl may be explored as a potential target for developing dsRNA-based biological pesticides for managing insect pests.

Xuekai Shi;Xiaojian Liu;Kristopher Silver;Kun Yan Zhu;Jianzhen Zhang

Institute of Applied Biology,Shanxi University,Taiyuan,Shanxi,China;College of Life Science,Shanxi University,Taiyuan,Shanxi,China;Department of Entomology,Kansas State University,Manhattan,Kansas,USA

中国昆虫科学（英文版）

[1]Xuekai Shi;Xiaojian Liu;Kristopher Silver;Kun Yan Zhu;Jianzhen Zhang-.Lethal giant larvae gene is required for normal nymphal development and midgut morphogenesis in Locusta migratoria)[J].中国昆虫科学（英文版）,2022(04):1017-1029

Lgl,tholog,LmLgl,Malpighian,dsLmLgl

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