Gut microbiota plays an important role in coronary heart disease,but its compositional and functional changes in unstable angina(UA)remain unexplored.We performed metagenomic sequencing of 133 newly diagnosed UA patients and 133 sex-and age-matched controls,and profiled the fecal and plasma metabolomes in 30 case-control pairs.The alpha diversity of gut microbiota was increased in UA patients:the adjusted odds ratios(ORs)per standard deviation increase in Shannon and Simpson indices were 1.30(95％confidence interval,1.01-1.70)and 1.36(1.05-1.81),respectively.Two common species(depleted Klebsiella pneumoniae and enriched Streptococcus parasanguinis;P≤0.002)and three rare species(depleted Weissella confusa,enriched Granulicatella adiacens and Erysipelotrichaceae bacterium 6_1_45;P≤0.005)were associated with UA.The UA-associated gut microbiota was depleted in the pathway of L-phenylalanine degradation(P = 0.001),primarily contributed by Klebsiella pneumoniae.Consistently,we found increased circulating phenylalanine in UA patients(OR = 2.76[1.17-8.16]).Moreover,Streptococcus parasanguinis was negatively correlated with fecal citrulline(Spearman's rs =-0.470,P = 0.009),a metabolite depleted in UA patients(OR = 0.26[0.08-0.63]).These findings are informative to help un-derstand the metabolic connection between gut microbiota and UA.

Department of Occupational and Environmental Health,Key Laboratory of Environment and Health,Ministry of Education and State Key Laboratory of Environmental Health(Incubating),School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China;Department of Cardiovascular Diseases,Sinopharm Dongfeng General Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China;Department of Medicine,Yong Loo Lin School of Medicine,National University of Singapore,Singapore 117599,Singapore;Department of Epidemiology and Biostatistics,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China

[1]Xuezhen Liu;Miaoyan Shen;Han Yan;Pinpin Long;Haijing Jiang;Yizhi Zhang;Lue Zhou;Kuai Yu;Gaokun Qiu;Handong Yang;Xiulou Li;Xinwen Min;Meian He;Xiaomin Zhang;Hyungwon Choi;Chaolong Wang;Tangchun Wu-.Alternations in the gut microbiota and metabolome with newly diagnosed unstable angina)[J].遗传学报,2022(03):240-248

Alternations,metabolomes,parasanguinis,adiacens

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